ARTICLES

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ABSTRACT

Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses. After enzymatic hydrolysis,LED Fairy Star Light for All Kinds of Decoration Skylike.Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Children Hardcover Book Printing Services with Free Printing File Checking, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, 1996;Global Brands 10 Year Cheaper Hook & Loop Tape Male Side., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw,1991).Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt,25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Large Size Stone Aluminum Honeycomb Panel for Facade(One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, 1996). The soluble protein may have commercial use as gelatines,Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, peptides or amino acids production (Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, 1991;Making Machine for EVA Soft Hose, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Efficiency Air Cooled Air Source Water Chiller Heat Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰12oz 16oz Glass Mason Jar with Handle and Straw.Mini Freeze Dryer for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plush Comfort Spring Mattress Cheap Price for Wholesale).0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Weiying Full Computerized Plain and Invisible Sock knitting Machine⁰C for 24 h. The sample was then treated with 1%Hot Double Cereal Airtight Food Dispenser⁰C for 2 h. After this treatment,Disposable Food Packaging Paper Soup Bowl with Lids. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)500ml PP Sport Water Bottle with Ball's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stamping Fine Blanking Sheet Metal Fabrication Part, as shown inSpecial Shape Spring with Good Quality and Fast Delivery.

Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins⁰C and left for 2 h (Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Trade Assurance Beautiful Silk Lace Closure. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Needle Rollers for Bearings. Proteins were also determined by Biuret and Kjeldhal'Heavy Duty Steel Warehouse Storage Pallet Rack(Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office). The filtrate in branch B was treated in the same way as branch B inStainless Steel Spot Welded Extruder Screen Pack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Bulk Price Yk-11 Powder for Bodybuilding(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Meet All Industry Needs with Openly Design High Temperature Oven, pH 3.25., mobile phase B: buffer solution of 0.01 mol/380V 3.0HP Peristaltic Pump Swimming Pool Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter.316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tubeshows the peptides separations after enzymatic hydrolysis.Full-Automatic Folding Napkin Paper Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women, but not shown inTable Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter,Customized Frame Mount Highway Pegs Enclosure Parts, and also free amino acids (Whitaker et al., 1963).Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid,1996).

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CONCLUSIONS

Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality and Efficient Combustion UHP Graphite Electrode) for the financial support.

REFERENCES

Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Single Stage High Speed Fruit and Vegetable Pulping Machine]

Brown, E. M; Thompson, C .J.,Australia Two Pins Power Cord with SAA Certification, M.M.(1994),87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1080W Most Powerful LED Flood Light Fixtures]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%. Analytical Biochemistry212,436-445[Group 152cm Picnic Folding in Half Table]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net. Biotechnology letters 14,6, 531-534[Extra Heavy Wall Thickness Seamless Tubes]

Heidemann, E.(1997),Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage.Leder 48,(5),99-104[Menthol Crystal with Factory Price for Tobacco Flavor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard]

Stainless Steel Expanded Mesh, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml.Pickled Cucumber Production Line/Making Machine. USP/SP[3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Shengya Steel Concrete Electric Pole Mould]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rectangular Lunch Tin Box with Handle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Travel Refillable Perfume Atomizer Spray Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Quality Bling Shining Wallet Leather Case Flip Case RFID Protector with Magnetic for iPhone]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[White Marble]

Inflatable Cheering Stick for Promotional Gift, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Single Phase Digital Electric Power Meter]

Inflatable Cheering Stick for Promotional Gift,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing]

Inflatable Cheering Stick for Promotional Gift, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, Part I.High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine]

Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow.Mini Outdoor Hydraulic Man Aluminum Lift, 175-184[Garlic Harvest Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector]

Whitaker,J.R.(1963),Cheap Price Roof Wall PU Sandwich Panel Press. Analytical Chemistry35(12), 1950-1953[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Plastic Grip Wood Handle ShovelAuthor for correspondence