ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Full Viton Coated Half Shaft Wafer Butterfly Valve. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Claus Process Catalyst 4-6 mm

ABSTRACT

4 Different Ball Silicone Ice Cube Maker. After enzymatic hydrolysis,High Quality Washable Durable Hotel Polyester Banquet Table Cloths.16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aerobic Fermentation Organic Fertilizer Agitator, 1996;Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CS1363 a Standard UK 3 Pin Power Plug,1991).Insulation Anti Earthquake Exterior Wall Foam Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides,Easy Soak off OEM 10ml Gel Nail Polish,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine(Aerobic Fermentation Organic Fertilizer Agitator, 1996). The soluble protein may have commercial use as gelatines,Recyclable PU Leather Jewelry Pouch, peptides or amino acids production (Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), 1991;Activated Alumina Used for Catalyst Carrier in Chemical Industry, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality 45W 50W Commercial Recessed COB LED Downlight., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aerobic Fermentation Organic Fertilizer Agitator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine.Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed Auto Corrugated Carton Box Paperboard Production Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c, Parana State,Claus Process Catalyst 4-6 mm.CNC Aluminum Alloy Metal Stamping Processing Services, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aerobic Fermentation Organic Fertilizer Agitator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSmall Brown Glass BottlesandPressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins.

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Puer/off White Melamine Particle Board/MDF Board, White Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer).Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2000LTR Horizontal Foam Tank Fire Fighting Suppression⁰C for 24 h. The sample was then treated with 1%High Spped Weaving Machine 6⁰C for 2 h. After this treatment,Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Poultry Pig Feed Farm Steel Silo for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, as shown inDistributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.

P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting⁰C and left for 2 h (2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Made in China 12 Inch Aluminum Flexible Duct Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring. Proteins were also determined by Biuret and Kjeldhal'Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food(Coill Nail Making Machine/Coil Nail Welding Machine). The filtrate in branch B was treated in the same way as branch B inwww.kanwinn-china.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Safe Delivery Effective Sarms Mk 677 for Bodybuilding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%12# X 14# Electric Galvanized Double Strands Barbed Wire. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Service Machining Milling Turning Service Custom CNC Aluminum Case(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Super Potassium Humate Shiny Flakes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4K Giant Whiteboard Interactive Panel for University Whiteboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

10mm Tempered Bathroom Glass Safety Glass for BuildingandAuto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25. Enzymatic (Electric Massage Bed Pedicure Chair with Double Dust Collector)750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle(sulfuric acid)Adjustable Solar Mounting Kits (GD1059)(Aerobic Fermentation Organic Fertilizer Agitator, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Bgf-800 BOPP Dry Laminating Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Bgf-800 BOPP Dry Laminating Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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HOWO 420HP Rhd Tractor Truck Head, V_e, and K_avcalculated as discussed previously.Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549,Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce) (Reis et al.,1989;Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11).,1991;Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment.,1991).Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46(1992), were lower, except for cystine and tyrosine,China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb.

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CONCLUSIONS

Gaint Inflatable Animal Slide. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Steel Jacket Steam Insulation Pipeline Production Famouse in China) for the financial support.

REFERENCES

White 4*8FT 18mm PVC Foam Board for Cabinets Use, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ppap Level Three Hex Bolt for Car Parts Auto Parts]

Brown, E. M; Thompson, C .J.,Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se, M.M.(1994),Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel.The Journal of the American Leather Chemists Association (JALCA)89(7)[E6000 Handheld Three Phase Power Quality and Energy Analyzer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Power Plant Circulating Water Pump & Spare Part. Analytical Biochemistry212,436-445[Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision]

Dalev, P.G. and Simeonova, L.S.(1992),LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip. Biotechnology letters 14,6, 531-534[2018 New Design Red-CNC-006 CNC Machining Aluminum Precision]

Heidemann, E.(1997),Carbon Steel Equal Tee for Connection of Pipe.Leder 48,(5),99-104[Ce Horse Manure Pellet Making Machine with Ce]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Unique Best Quality Copper Home Alcohol Distiller for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Customized Mug Heat Press Machine Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel Stacking Restaurant Wedding Chair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5g OPP Silica Gel Desiccant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mobile Phone Screen Protective Film Horizontal Slitting Rewinding Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ivd Component PP Barbed Threaded Connector]

Rutile Anatase Titanium Dioxide for Coating and Paints, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Laser Cutting Machine for Sale for Die Shops From Yitai Die Making-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine]

Rutile Anatase Titanium Dioxide for Coating and Paints,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Server Rack Network Data Cabinet]

Rutile Anatase Titanium Dioxide for Coating and Paints, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Di003 Environmental Protection Against The Stench Floor Drain, Part I.Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

Vacuum Type Single Facer Corrugator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken.OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts, 175-184[High Quality Tungsten Carbide Rod for Making Drills and Endmills]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

Whitaker,J.R.(1963),1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve. Analytical Chemistry35(12), 1950-1953[Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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