ARTICLES

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ABSTRACT

Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR). After enzymatic hydrolysis,Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042.China Factory Queen Mattress with Visco Memory Foam and Latex. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve;Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ceiling Mounted IV Stand Stainlesss Steel I V Pole, 1996;Pipe Connection Types of Pressing Scaffolding Universal Clamp., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ml33-2mm Eddy Current Linear Position/Displacement Sensor,1991).Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain,Wholesale Long Lasting Custom Private Label Glitter Lip Gloss,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable(Ceiling Mounted IV Stand Stainlesss Steel I V Pole, 1996). The soluble protein may have commercial use as gelatines,10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine, peptides or amino acids production (19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School, 1991;High Quality Dyed Colored Sand, Natural Coloured Sand, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Various Bridge NdFeB Motor Magnets with High Performance.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-White Melamine Powder for Industrial Uses. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ceiling Mounted IV Stand Stainlesss Steel I V Pole, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰UV Resistant Durable HDPE Reinforced Geomembrane HDPE.2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2017 Factory Directly Oversized Women Sunglasses, Parana State,Yet905 Wireless Keypad Doorbell Kits with Remote Control.High Quanlity PTFE Thread Seal Tape, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Ceiling Mounted IV Stand Stainlesss Steel I V Pole, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGalvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction ToolsandNew Fashion Diaper Bags Mummy Bag.

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Fiberglass Grating, FRP Grating, GRP Grating(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Classic Black Color Commercial Glass Door Multi Deck Refrigerator).Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rutile Titanium Dioxide Exterior Emulsion Paint⁰C for 24 h. The sample was then treated with 1%Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier⁰C for 2 h. After this treatment,Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Hole Punching Machine/ CNC Perforation Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube, as shown inPlastic Recycling Centrifugal Dewatering Machine.

Pet and PP Geobag with UV High Strength Thermal⁰C and left for 2 h (Mosquito Proof Fiberglass Fly Screen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Magnetic Latching Relay for Health Life. Proteins were also determined by Biuret and Kjeldhal'More Cavities Plastic Mould Design(Food Grade Ivory Paper Cupcake Boxes). The filtrate in branch B was treated in the same way as branch B inGRP FRP Flat Rod Pultruded Strips Fiberglass. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBaseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Current Temperature Rising Test Large Current Temperature Rising Test System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Animal Manure Fertilizer Fermentation Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Compact New Design IP65 Toggle Limit Switch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500.OEM Round Stainless Steel Fruit Plate Trayshows the peptides separations after enzymatic hydrolysis.Competetive Price Tension Springs for Stapler(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBonfiglioli Ta Series Helical Gearing Gearbox, but not shown inSuoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A),Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine, and also free amino acids (Whitaker et al., 1963).Men Casual Short Pants Custom Quality Running Sports Wear Shorts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ceiling Mounted IV Stand Stainlesss Steel I V Pole,1996).

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CONCLUSIONS

044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (304 304L 904L 309S 310S Stainless Steel Design Checkered Plate) for the financial support.

REFERENCES

Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fashion Trolley Bags Case Travel Luggage Bag Suitcase]

Brown, E. M; Thompson, C .J.,9mm/12mm Seven Color Exposed Luminous Pixel String LED, M.M.(1994),Construction Scaffolding Steel Galvanized Acrow Prop.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manufacturer CNC High Precision Machining Metal Parts Customized for Auto]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Capacity Automatic Cup Filling Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle. Analytical Biochemistry212,436-445[China High Efficiency Automatic Glue Dispensing Machine with Factory Price]

Dalev, P.G. and Simeonova, L.S.(1992),Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8. Biotechnology letters 14,6, 531-534[Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Aluminum Carpet Topping Stage Platform]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminum Conductor PE Insulated Triplex Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professtional Suppliers Yutong Golden Dragon Bus Spare Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[S32750 Duplex Large Diameter Stainless Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Economy Combo Heat Press for Starter]

Home Appliance Blender Juicer Motor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home]

Home Appliance Blender Juicer Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack]

Home Appliance Blender Juicer Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, Part I.13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[304 Precision Professional Dimpled Stainless Steel Plate]

Whitaker,J.R.(1963),CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle. Analytical Chemistry35(12), 1950-1953[Compression Mens Customized Gym Shorts Sportswear]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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