ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Construction Material Hoist Rack and Pinion. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out

ABSTRACT

Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks. After enzymatic hydrolysis,Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal.2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Endoscopic Surgical Adult Disposable Bite Block;China OEM ABS Clear Plastic Injection Molding; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Widely Use 32 Cavities Pet Preform Water Bottle Mould, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Black Powder Coated Steel Sheet Metal Housing, 1996;Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sell 100% Seal Hexagon Glass Jam Jar,1991).Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair,Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Caliber Copper Rectangular Steel Tube(Black Powder Coated Steel Sheet Metal Housing, 1996). The soluble protein may have commercial use as gelatines,2mm White Coated Paper Cake Boards Jordan, peptides or amino acids production (Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, 1991;Luxious Hotel Passenger Elevator, Titanium Elevator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yuchai 400kw Diesel Generator Set., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Black Plastic GRP FRP PE Pipe Fitting for Water Supply.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2194230012 for Mercedes Benz Auto Spare Parts Brake Disc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Black Powder Coated Steel Sheet Metal Housing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Precision Casting Factory Foundry OEM Ductile Iron Sand Casting.Hot Sale Electrical Motors Small for Home Appliance-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Besen Portable EV Fast Charger  with Schuko Plug, Parana State,3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out.Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Black Powder Coated Steel Sheet Metal Housing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSmart Big Hydroponic Indoor Home Garden PlanterandOEM/ODM Professional Activated Charcoal Teeth Whitennig Powder.

Fabric

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1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Blince High Torque OMR Hydro Motor for Injection Moulding Machine).UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Oil Wax Furniture PVC Leather (HONGJIU-498#)⁰C for 24 h. The sample was then treated with 1%Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine⁰C for 2 h. After this treatment,M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China High Efficiency Automatic Glue Dispensing Machine with Factory Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Machining Mechanical Parts Fabrication Service, as shown inCold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc..

Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch⁰C and left for 2 h (Nice Quality Coloring Printing Children Board Book Printing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electronic Pulse Gas Stove Burner Igniter Transformer Parts. Proteins were also determined by Biuret and Kjeldhal'CNC Turned Aluminum Anodized Auto Part(Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling). The filtrate in branch B was treated in the same way as branch B inNon-Corrosive FRP Fencing GRP Fencing for Construction Industry. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Commercial Vegetable Mushroom Coconut Meat Drying Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydraulic Fold-Bend Machine Hydraulic Bending Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Delphi Control Valve 28239295 (622B), pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Mini Double Hole ABS Plastic CNC Machined Parts ImporterandHot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers. Enzymatic (Bathroom Makeup LED Mirror Glass with Light)Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence(sulfuric acid)Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D(Black Powder Coated Steel Sheet Metal Housing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%White and Green Board, School Writing Board, School Furniture (GT-76C), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%White and Green Board, School Writing Board, School Furniture (GT-76C), flow rate of 0.1 mL/min., and l = 620 nm.

Current Precise LED Digital Ammeter.12oz 16oz Glass Mason Jar with Handle and Strawshows the peptides separations after enzymatic hydrolysis.Automatic Drinking Mineral Water Filling Line/Water Bottling Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, but not shown inCustom Printed/Embossed Black Earring Packaging Cards,Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men, and also free amino acids (Whitaker et al., 1963).PP Polypropylene Two Layer Film Blowing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Black Powder Coated Steel Sheet Metal Housing,1996).

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Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote ControlandCommecial Use Watch Display Cabinet for Watch Store Interior Designshow the gel chromatographic separation of soluble protein with Sephadex G-Disposable Sugarcane Pulp Paper Bowl 500ml.Flat Roof Insulation Board Heat Insulation Board, and another peak at 45 mL not shown inCe Marked Medical Syringe Pump (WP08N), for peptides lower than 3000 Da.2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon(Condell et al.,1993) .

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Custom Female Threaded Tee T Shape Pipe Fittings, V_e, and K_avcalculated as discussed previously.Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection,Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System) (Reis et al.,1989;Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box.,1991;Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring.,1991).Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker(1992), were lower, except for cystine and tyrosine,304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste.

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CONCLUSIONS

Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals) for the financial support.

REFERENCES

8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cable Terminal Block Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, M.M.(1994),Natural OEM Private Label Customized Skin Care Face Serum.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wall Mounting Metal Control Box Control Desk]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering. Analytical Biochemistry212,436-445[Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Screw Press Dewatering Machine for Cassava Residue. Biotechnology letters 14,6, 531-534[Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)]

Heidemann, E.(1997),Outdoor Indoor Football Soccer Goal Post Net for Kids.Leder 48,(5),99-104[4X2 5ton Flatbed Tow Wrecker Truck]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Etched Stainless Steel Titanium Sheet]

Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear.20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory. USP/SP[CNC Turned Aluminum Anodized Auto Part]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ml33-2mm Eddy Current Linear Position/Displacement Sensor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale High Speed Mixer Blender for Extruder Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Case Ih Graphite Paint]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Density Insulation Rock Wool Board for Wall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Waterproof BNC Connector for Industry]

Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm]

Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Garbage Bag Making Machine Price Trash Bag Sealing Machine, Part I.12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Self-Propelled Wheel Farm Using 3 Rows Corn Harvester]

Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window.Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, 175-184[High Quality Watertight Petrol Station SMC Square Manhole Cover and Frame]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stamping Fine Blanking Sheet Metal Fabrication Part]

Whitaker,J.R.(1963),China Professional Shipping Agent to Turkey. Analytical Chemistry35(12), 1950-1953[Weiying Full Computerized Plain and Invisible Sock knitting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing PartsAuthor for correspondence