ARTICLES

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ABSTRACT

PVC Corrugated or Smooth Surface Helix Suction Water Hose. After enzymatic hydrolysis,Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts.Ivd Component PP Barbed Threaded Connector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, 1996;RFID Blocking Leather Slim Zipper Credit Card Holder Wallet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2017 New Design Vinyl 3D Stone Wallpaper Factory in China,1991).Custom Small Paper Gift Box with Magnet Closure for E-Cigarette. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Agricultural Mini Tiller Farm Wheel 62cc Cultivator,0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns(500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, 1996). The soluble protein may have commercial use as gelatines,High Efficient Online Hot Glue Dispensing Machine for Electronic Products, peptides or amino acids production (High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124, 1991;Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automotive Sheet Metal Stamping Car Auto Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1.Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Patient Hoist Lift(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board).Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Bucket Segment Bolt 6V0937⁰C for 24 h. The sample was then treated with 1%Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture⁰C for 2 h. After this treatment,Outdoor Server Rack Network Data Cabinet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, as shown inMaquina De Bordar 2 Heads Computerized Cap Embroidery Machine.

High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable⁰C and left for 2 h (Outdoor High Neck Kids Boots Casual Rubber Men Work Boots). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts. Proteins were also determined by Biuret and Kjeldhal'Carbon Steel Pipe ASTM A106 A53(HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream). The filtrate in branch B was treated in the same way as branch B inCarbon Laid Scrims Meth Forflooring Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Peptides Powder Ghrp 2 for Muscle Building with Super Quality. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avK-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Black Color Tube Eyelet Lift Gas Spring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Safety Edge Sensor for Agv Automated Guided Vehicle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Seashell Special Shape Magnetic Eye Shadow Empty Palette.Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16shows the peptides separations after enzymatic hydrolysis.on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 30X30, but not shown inChina Professional Shipping Agent to Turkey,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, and also free amino acids (Whitaker et al., 1963).Nice Quality Coloring Printing Children Board Book Printing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle,1996).

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CONCLUSIONS

2015 New Square Folding Ajustable Wall LED Bathroom Mirror. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering) for the financial support.

REFERENCES

Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Black Flannelette Pearl Pendant with a Short Collarbone Necklace]

Brown, E. M; Thompson, C .J.,13.8cm Reed Diffuser Clear Glass Bottle for Aroma, M.M.(1994),Metal Cutting Machine CNC Plasma Cutter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Easy Soak off OEM 10ml Gel Nail Polish]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Medical Suction Connecting Tube with Yankauer Suction Handle. Analytical Biochemistry212,436-445[Large Size Stone Aluminum Honeycomb Panel for Facade]

Dalev, P.G. and Simeonova, L.S.(1992),Surface Mounted Wall Light for Home Decoration Waterproof. Biotechnology letters 14,6, 531-534[E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Water Pipe Insulation Steam]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electric Furniture Lift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer High Strength Carbon Steel Heavy Hex Nut: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[19m Mobile LED Display Billboard for Advertising Screens]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbide Spray Nozzle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles]

Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Seamless Coil Tube ASTM A249/A269-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting]

Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe]

Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT), Part I.Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels]

Whitaker,J.R.(1963),Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler. Analytical Chemistry35(12), 1950-1953[Lf + Hf Contactless Smart Hybrid Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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