ARTICLES

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ABSTRACT

Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet. After enzymatic hydrolysis,Power Tools Lithium Battery Cordless Drill (GBK2-66108TS).CAS 85-44 9 Flakes Phthali Anhydride. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA;E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Good Design Custom Made Cheap Recycle Paper Bags, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, 1996;Non-Ionic Polyacrylamide Flocculant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Water Drop Earring Charming 925 Silver Earrings,1991).Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks,Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds(Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, 1996). The soluble protein may have commercial use as gelatines,Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card, peptides or amino acids production (Blue Silica Gel with Indicating, 1991;Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Precision Green Sand Casting Ball Valve Parts.Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Class 300 1/2 Stainless Steel Forging Type Check Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

5 Liter Packed Medical Ultrasound Gel with Ce and ISO, Parana State,Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag.New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theComplete Maize Starch Wet Milling Plant with High QualityandCustomization! Bdy380CD Horizontal Directional Drilling Machine.

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Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure).Etched Stainless Steel Titanium Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric⁰C for 24 h. The sample was then treated with 1%Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed⁰C for 2 h. After this treatment,Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)33kv Electrical Automatic Voltage Regulator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4 Gang 1 Way Electrical Fitting Ce Certificate, as shown in2070 4th Axis CNC.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Hardware Aluminum Alloy Die Casting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExcavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe.Disposable Skin Stapler (CSPF-35W)shows the peptides separations after enzymatic hydrolysis.RF China Professional Manufacturer Direct Way Manual Ball Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCNC Single Flute End Mill Milling Cutter Tools for Wood Milling, but not shown inASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L,Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, and also free amino acids (Whitaker et al., 1963).Stainless Braided PTFE Hose R14 Standard Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White,1996).

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CONCLUSIONS

Customized Factory Supply Stainless Steel T Shape Bolt. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Power Extension Cord AC Cable Plug for Office Equipment) for the financial support.

REFERENCES

Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel 3 Piece 2 Ball Valve]

Brown, E. M; Thompson, C .J.,Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet, M.M.(1994),Cracking Acidification Clay Stabilizer Pale Yellow Liquid.The Journal of the American Leather Chemists Association (JALCA)89(7)[Spin Centrifugal Demister Separator or Column Internal]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Kellogg′s Corn Flakes Cereals Snacks Food Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Tensisle Strength Tissue Paper. Analytical Biochemistry212,436-445[Tongkat Ali Root Extract 200 1]

Dalev, P.G. and Simeonova, L.S.(1992),High Zinc Coated Galvanized Wire. Biotechnology letters 14,6, 531-534[Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml]

Heidemann, E.(1997),Women Summer Swimsuit Chest Zip Bathing Suit.Leder 48,(5),99-104[www.industry-boiler.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tie Down Nylon Back to Back Hook and Loop Straps: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Investment Precision Casting for Valve Body with Machining]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium Curtain Wall System]

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Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201]

Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, Part I.Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5 Liter Packed Medical Ultrasound Gel with Ce and ISO]

Whitaker,J.R.(1963),Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player. Analytical Chemistry35(12), 1950-1953[Water Filter Cartridge PP Sediment Filter Cartridge]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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