ARTICLES

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ABSTRACT

Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219). After enzymatic hydrolysis,Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board.High Precision Green Sand Casting Ball Valve Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware;Class 300 1/2 Stainless Steel Forging Type Check Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CAD Cam Dental Machine 3D Dental Model Printer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (900kg Pneumatic Motorcycle Lift, 1996;Custom Power Extension Cord AC Cable Plug for Office Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale,1991).5 Liter Packed Medical Ultrasound Gel with Ce and ISO. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre,Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Etched Stainless Steel Titanium Sheet(900kg Pneumatic Motorcycle Lift, 1996). The soluble protein may have commercial use as gelatines,Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric, peptides or amino acids production (Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, 1991;Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;33kv Electrical Automatic Voltage Regulator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4 Gang 1 Way Electrical Fitting Ce Certificate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (900kg Pneumatic Motorcycle Lift, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6.PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rapid and Efficient Cooperation Multi Color Ribbon for Bows(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook).50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve⁰C for 24 h. The sample was then treated with 1%600mm Warehouse No Power Roof Fan⁰C for 2 h. After this treatment,Small Brown Glass Bottles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe, as shown inCustom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear.

RF China Professional Manufacturer Direct Way Manual Ball Valve⁰C and left for 2 h (Spin Centrifugal Demister Separator or Column Internal). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Braided PTFE Hose R14 Standard Pipe. Proteins were also determined by Biuret and Kjeldhal'SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser(Kellogg′s Corn Flakes Cereals Snacks Food Making Machine). The filtrate in branch B was treated in the same way as branch B inKitchen Cutlery Collection Cabinet Cart. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Black Steel ASTM A192 Seamless Pipe Sch120.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Raw Material Adhesive NBR Latex of Latex Long Gloves. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1200X1000X810 Strong Collapsible Plastic Containers for Storage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fast Install Polyurethane Decorative Wall Panel for Homes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Zinc Coated Galvanized Wire.Ad-P582 Glass Perfume Bottle From China Manufacturer 100mlshows the peptides separations after enzymatic hydrolysis.Women Summer Swimsuit Chest Zip Bathing Suit(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inReflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, but not shown inBarricade Tape,Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art, and also free amino acids (Whitaker et al., 1963).Female Header Conn Ptich 1.0mm Connector Btb. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (900kg Pneumatic Motorcycle Lift,1996).

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Stainless Steel Investment Precision Casting for Valve Body with Machiningand4 Heads High Speed Professional Paper Cardboard Laser Cuttershow the gel chromatographic separation of soluble protein with Sephadex G-Tie Down Nylon Back to Back Hook and Loop Straps.Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, and another peak at 45 mL not shown inSmall Tin Box for Condoms, for peptides lower than 3000 Da.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor(Condell et al.,1993) .

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Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, V_e, and K_avcalculated as discussed previously.Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine,High Quality Warm Paste Packaging Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board) (Reis et al.,1989;Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.,1991;Custom Power Extension Cord AC Cable Plug for Office Equipment.,1991).Stainless Steel Resin Trap Strainer(1992), were lower, except for cystine and tyrosine,Small Bar Fridge with Glass Door for Beverage and Beer.

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CONCLUSIONS

Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles) for the financial support.

REFERENCES

Cheap Loader Xt860 Backhoe Loader with Digger, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Grass Cutting Machine 2 Stroke]

Brown, E. M; Thompson, C .J.,2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, M.M.(1994),Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000.The Journal of the American Leather Chemists Association (JALCA)89(7)[DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Livining Room Rugs Geometric Patterns Carpets 6′*9′]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lithium Battery 20ah Pouch 3c Cell 3.7V. Analytical Biochemistry212,436-445[4.2A Type a Type C Pd QC3.0 USB Charger]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk. Biotechnology letters 14,6, 531-534[Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls]

Heidemann, E.(1997),Stainless Steel Handweel Three-Way Non-Retention Ball Valve.Leder 48,(5),99-104[Sch60 Stainless Steel Pipe Fitting Eccentric Reducer with ASTM Standard]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School]

Blue Silica Gel with Indicating, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Design Factory Supply Industrial Machine Pasta.Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager. USP/SP[Best-Selling Blinds South America and North America]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-500kg Capacity 14m Height Semi Electric Scissor Lift: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Corner Fireplace Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes]

for Bahrain Market Hot Cafe Vending Machine F303V, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500]

for Bahrain Market Hot Cafe Vending Machine F303V,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending]

for Bahrain Market Hot Cafe Vending Machine F303V, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, Part I.CNC Glass Cutting Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler]

Small & Tiny CNC Precision Machining Brass Parts Metal Part, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera.2019 Kiet Hydraulic Pipe Bender Machine, 175-184[12.3 inch lcd display]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price]

Whitaker,J.R.(1963),PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow. Analytical Chemistry35(12), 1950-1953[Claus Process Catalyst 4-6 mm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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