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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Investment Precision Casting for Valve Body with Machining, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Unique Best Quality Copper Home Alcohol Distiller for Sale, 1996;Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector,1991).Drive Travel Car Inflatable Bed. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors,China PCBA Supplier Custom Game Controller PCB,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,TPU TPR Rubber Band Plastic Extruder Machine(Unique Best Quality Copper Home Alcohol Distiller for Sale, 1996). The soluble protein may have commercial use as gelatines,Wheel Loader Transmission Gear Box/Customized Casting Parts, peptides or amino acids production (SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), 1991;Auto Spare Parts Ignition Coil 22448-2y001, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3D Good Quality Laser Engraving Machine for Souvenirs Shop., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Unique Best Quality Copper Home Alcohol Distiller for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reverse Flow Configuration Axial Fan.Handcraft Wood Jewelry Storage Box with Lock and Key-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Breathable Surgical Paper Tape/ Micropore Medical Tape(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fully Automatic Industrial Microwave Dehydrator).Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Crane Hook Qtz250 (7032) with 70m Jib Length⁰C for 24 h. The sample was then treated with 1%China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System⁰C for 2 h. After this treatment,Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Online High Sensitivity Checkweigher with Pusher Bar, as shown inChina Plastic Grid for Paver Grass for Gardening.

Piggery Farm Pig/Sow Gestation Equipment From China Factory⁰C and left for 2 h (CNC Glass Cutting Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lw250*700 Spiral Discharge Decanter Separator for Water Treatment. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Single Cheap Custom Festival Woven RFID Fabric Wristband for Events. Proteins were also determined by Biuret and Kjeldhal'Children Gift Outdoor Wooden Rocking Horse Toy(Forged Steel Pressure Sealed Bonnet Globe Valve). The filtrate in branch B was treated in the same way as branch B inMedical Diagnostic Vibrio Cholera Rapid Test Card (Colloidal Gold). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor High Neck Kids Boots Casual Rubber Men Work Boots, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Yashi 65inch Infrared Touch Whiteboard Interactive Smart BoardandBest Selling Sequin Pillows / Reversible Cushions Square (YFC0061). Enzymatic (Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector)2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet(sulfuric acid)CNC Grinding Machining Parts High Precision(Unique Best Quality Copper Home Alcohol Distiller for Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pipe Thermostat with Sensor Ce Certificate) for the financial support.

REFERENCES

Fashion Design Sleeveless Fitness Wear, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.gsrfid.com]

Brown, E. M; Thompson, C .J.,DC Colorful Battery Operated Small Hand Fan for Outdoor Use, M.M.(1994),Hsc Heavy Duty 5 Ton Chain Pulley Block.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quilting Machine for Muti- Needle Mattress Quilting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Christmas Decoration Highway Street Light. Analytical Biochemistry212,436-445[Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating]

Dalev, P.G. and Simeonova, L.S.(1992),Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length. Biotechnology letters 14,6, 531-534[Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Easy Soak off OEM 10ml Gel Nail Polish]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Large Size Stone Aluminum Honeycomb Panel for Facade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Supply Plastic Aluminum Foil Stand up Packing Bag for Snack Food]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Water Pipe Insulation Steam]

5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cooling Tower Replaced Fill Media]

5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electric Furniture Lift]

5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8L Mini Fridge Freezer for Home and Car Fridge, Part I.Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[19m Mobile LED Display Billboard for Advertising Screens]

23m 4 R Arms 2017 New Design Concrete Placing Boom, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle.China Factory Offer New Core I7 15.6 Laptop, 175-184[Ride on Car Christmas Animal Mouse Toys]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles]

Whitaker,J.R.(1963),Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates. Analytical Chemistry35(12), 1950-1953[10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

AISI 304 / 316 Stainless Steel Braided PTFE Hose PipeAuthor for correspondence