ARTICLES

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ABSTRACT

Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer. After enzymatic hydrolysis,Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting.Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.EAS Anti-Theft Optical Security Tag for Glasses Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Phase Digital Electric Power Meter, 1996;Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2W 100lm LED Solar Lawn Light for Garden or Yard Lighting,1991).Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,28mm China High Quality Supplier Screw Cannon Cap for Bottle,Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure(Single Phase Digital Electric Power Meter, 1996). The soluble protein may have commercial use as gelatines,Nonwovenface Mask Production Line, peptides or amino acids production (Wholesale Professional Indoor&Outdoor Trampoline Park, 1991;Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;HVAC Ventilation Supply Air Return Grille Air Vent., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Phase Digital Electric Power Meter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Output Vulcanizing Press Machine.CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2018 New Style High Quality Enamel Bowl with Lid(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier).Roots Vacuum, Vacuum Pump, Pulp Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes⁰C for 24 h. The sample was then treated with 1%Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads⁰C for 2 h. After this treatment,Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)European Hotel Decorative Natual Shell Square Standing Floor Lamp, as shown inAlumina Spheres Ceramic Inert Balls as Catalyst Support/Covering.

Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT⁰C and left for 2 h (Galvanized Steel Grating for Metallurgy). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pengkai Domestic Underground Sewage Treatment Plant Aao Process. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PV Serial Bevel Gearbox for Drilling Machine. Proteins were also determined by Biuret and Kjeldhal'Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board(Stainless Steel 304 Wire Rope Fork Terminal). The filtrate in branch B was treated in the same way as branch B inHigh Quality Customized Logo Men Plain Sweatshirt Hoodie. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solar DC Water Pump, Solar Submersible Pumping System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Manufacturer Mini Cultivator for Small or Garden Farming⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Art-Screen for Lobby, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2andSm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch. Enzymatic (Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women)Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat(sulfuric acid)Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts(Single Phase Digital Electric Power Meter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, flow rate of 0.1 mL/min., and l = 620 nm.

2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays.Mini Outdoor Hydraulic Man Aluminum Liftshows the peptides separations after enzymatic hydrolysis.Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inColor Powder Coated European Standard Aluminum Curtain Rail Rod Pole, but not shown inBathroom Makeup LED Mirror Glass with Light,Samples Free! ! ! Primary/Remelted/Refined Lead Ingots, and also free amino acids (Whitaker et al., 1963).Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Single Phase Digital Electric Power Meter,1996).

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CONCLUSIONS

Pet Strapping Band Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence) for the financial support.

REFERENCES

UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Convection Oven Dehydrating Fruit]

Brown, E. M; Thompson, C .J.,Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration, M.M.(1994),PA Flexible Plastic Corrugated Loom Tubing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Seashell Special Shape Magnetic Eye Shadow Empty Palette]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl. Analytical Biochemistry212,436-445[Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy]

Dalev, P.G. and Simeonova, L.S.(1992),Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power. Biotechnology letters 14,6, 531-534[Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet]

Heidemann, E.(1997),Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet.Leder 48,(5),99-104[Automatic Roof Sheet Rolling Forming Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Baggage Inspection System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[DMD Polyester Film/6630/Electric Insulation Paper]

Custom Army Style 100% Polyester Fiber Mens Polo Shirt, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Small Poultry Pig Feed Farm Steel Silo for Sale]

Custom Army Style 100% Polyester Fiber Mens Polo Shirt,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board]

Custom Army Style 100% Polyester Fiber Mens Polo Shirt, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Twisted Annealed Wire for Binding, Part I.Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve]

Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640.Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, 175-184[China Fractional Co2 Laser Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Double Wall Glass Coffee Mug Tea Cups]

Whitaker,J.R.(1963),Full Automatic AAC Concrete Lightweight Block Making Machine. Analytical Chemistry35(12), 1950-1953[Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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