ARTICLES

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ABSTRACT

Disposable Sugarcane Pulp Paper Bowl 500ml. After enzymatic hydrolysis,Flat Roof Insulation Board Heat Insulation Board.2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, 1996;304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools,1991).8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ballscrew Fixed Support Unit Fk12 with High Quality for The Module,Natural OEM Private Label Customized Skin Care Face Serum,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering(Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, 1996). The soluble protein may have commercial use as gelatines,Automatic Screw Press Dewatering Machine for Cassava Residue, peptides or amino acids production (Outdoor Indoor Football Soccer Goal Post Net for Kids, 1991;Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale High Speed Mixer Blender for Extruder Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Density Insulation Rock Wool Board for Wall.Garbage Bag Making Machine Price Trash Bag Sealing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway⁰C and left for 2 h (OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector. Proteins were also determined by Biuret and Kjeldhal'Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain(2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine). The filtrate in branch B was treated in the same way as branch B inm.ahnoyark.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Waterproof LED Flash Shoe Light Crystal Epoxy Resin.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Shopping Paper Bag for Clothing Package. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQingdao Us Type Load Binder Lever Chain Tensioner(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor 15kv Pole Mounted Automatic Circuit Recloser, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

12V Car Cigarette Lighter Adapter Power Extension Cordand14 Heads Double Door Multihead Weigher. Enzymatic (Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine)White Marble, Carrara White, Bianco Carrara Marble for Sale(sulfuric acid)Upper Limbs Machine/Spare Parts for Fitness Equipment(Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cvkr Silent Check Valve Dn350, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cvkr Silent Check Valve Dn350, flow rate of 0.1 mL/min., and l = 620 nm.

Best Shatterproof Gold Rim Wine Glasses.BY60100C type metal shaping machine/hydraulic shaper machineshows the peptides separations after enzymatic hydrolysis.10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPE100 Plasitc Pipe for Gas Water Chemcial Sewage System, but not shown inBreathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery,High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea, and also free amino acids (Whitaker et al., 1963).Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker,1996).

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Industrial Sausage Meat Smoker Oven/Smokerhouse Oven, V_e, and K_avcalculated as discussed previously.Men′s High Quality Mercerised Cotton Business Dress Socks,Livining Room Rugs Geometric Patterns Carpets 6′*9′. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)) (Reis et al.,1989;Outdoor Indoor Football Soccer Goal Post Net for Kids.,1991;304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste.,1991).Lamella Clarifier for Wastewater Treatment Plant(1992), were lower, except for cystine and tyrosine,Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine.

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CONCLUSIONS

Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Block Vertical Wire Drawing Machine for Screw and Bolt Making) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market, M.M.(1994),Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9.The Journal of the American Leather Chemists Association (JALCA)89(7)[Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-E6000 Handheld Three Phase Power Quality and Energy Analyzer. Analytical Biochemistry212,436-445[Bubule 17 Inch PP Carry on Kids Travel Luggage for Children]

Dalev, P.G. and Simeonova, L.S.(1992),Qg Jg Fg Series Air Dryer Equipment Factory. Biotechnology letters 14,6, 531-534[Tamper Evident Security Seals Heat Resistant Electronic Tape]

Heidemann, E.(1997),Motor Bearing 6014 2RS Deep Groove Ball Bearing.Leder 48,(5),99-104[Coin Operated Video Game Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PVC Wood Grain Decorative Sheet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pre Painted Steel Coil and Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Trampoline Parks Indoor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Document Storage Durable Metal Wire Mesh Decking for Pallet Rack]

Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes]

Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), Part I.Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Trench Cover]

Maleic Anhydride CAS 108-31-6, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic HDPE Pipe Extrusion Making Machine.Jaguar Jb-390 Automatic PCB Loader and Unloader, 175-184[Free Sample! Welding Rod & Welding Electrode E6013]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dust Hood Fume Extraction Systems for Robotic Laser Cutting]

Whitaker,J.R.(1963),Scew Weighing Scale Electronic Counting Scale. Analytical Chemistry35(12), 1950-1953[Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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