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ABSTRACT

High Quality and Efficient Combustion UHP Graphite Electrode. After enzymatic hydrolysis,Graphite Ring Good Lubrication.Flat Head Six-Lobe with Gasket Self Tapping Screws. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw;Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Weiying Full Computerized Plain and Invisible Sock knitting Machine, 1996;Hot Double Cereal Airtight Food Dispenser., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Disposable Food Packaging Paper Soup Bowl with Lids,1991).500ml PP Sport Water Bottle with Ball. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stamping Fine Blanking Sheet Metal Fabrication Part,Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Trade Assurance Beautiful Silk Lace Closure(Weiying Full Computerized Plain and Invisible Sock knitting Machine, 1996). The soluble protein may have commercial use as gelatines,High Quality Needle Rollers for Bearings, peptides or amino acids production (Heavy Duty Steel Warehouse Storage Pallet Rack, 1991;Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Bulk Price Yk-11 Powder for Bodybuilding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Weiying Full Computerized Plain and Invisible Sock knitting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer.Meet All Industry Needs with Openly Design High Temperature Oven-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:380V 3.0HP Peristaltic Pump Swimming Pool Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Anti-Corrosion Fiberglass Sheet and Roll, Parana State,CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved.Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Weiying Full Computerized Plain and Invisible Sock knitting Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theP4.75 Indoor Single Dual Color Advertising LED Display BillboardandChina Cheap Newest 70*77cm XPE Foam 3D Wallpaper.

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Full-Automatic Folding Napkin Paper Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Geotextile for Airport Runways/High Way).Customized Frame Mount Highway Pegs Enclosure Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling⁰C for 24 h. The sample was then treated with 1%10mm Tempered Bathroom Glass Safety Glass for Building⁰C for 2 h. After this treatment,3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cattle Feed Factory Used Dust Collector and Filter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, as shown inCold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443).

Group 152cm Picnic Folding in Half Table⁰C and left for 2 h (Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tactile Custom Branded Membrane Switch Keyboard with Foil. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm. Proteins were also determined by Biuret and Kjeldhal'Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America(High Sensitivity Ground Metal Detector Searching for Treasure). The filtrate in branch B was treated in the same way as branch B inBook Bag for Students Children Kid Backpack for School. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAustralia Two Pins Power Cord with SAA Certification(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Anti-Corrosion Fiberglass Sheet and RollandFrosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml. Enzymatic (Pickled Cucumber Production Line/Making Machine)Rectangular Lunch Tin Box with Handle(sulfuric acid)Inflatable Cheering Stick for Promotional Gift(Weiying Full Computerized Plain and Invisible Sock knitting Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, flow rate of 0.1 mL/min., and l = 620 nm.

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industrial aluminum foil roll 8011 8079 1235, V_e, and K_avcalculated as discussed previously.Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval,High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ivd Component PP Barbed Threaded Connector) (Reis et al.,1989;Heavy Duty Steel Warehouse Storage Pallet Rack.,1991;Hot Double Cereal Airtight Food Dispenser.,1991).Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet(1992), were lower, except for cystine and tyrosine,Afraic Market Mobile Food Vending Cart.

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CONCLUSIONS

Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery) for the financial support.

REFERENCES

Double Wall Glass Coffee Mug Tea Cups, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Glass-Lined Equipment From 50 to 30000L]

Brown, E. M; Thompson, C .J.,Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning, M.M.(1994),30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Vibrating Condom Ring and Cock Ring Supplier. Analytical Biochemistry212,436-445[Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper]

Dalev, P.G. and Simeonova, L.S.(1992),Full Color Print Glossy Laminated PP Woven Shopping Gift Bag. Biotechnology letters 14,6, 531-534[Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system]

Heidemann, E.(1997),High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40.Leder 48,(5),99-104[Concrete Steel Frame Formwork]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Medical Packing Self Sealing Sterilization Pouch]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[380V 3.0HP Peristaltic Pump Swimming Pool Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Steel Tube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length]

Wall Mounting Metal Control Box Control Desk, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Precision Tooling Grinding Machine Part-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[45ton Proof Load Testing Water Bag for Life Boat]

Wall Mounting Metal Control Box Control Desk,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Chubby 163cm Fat Big Ass Female Real Sex Doll for Man]

Wall Mounting Metal Control Box Control Desk, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, Part I.Kellogg′s Corn Flakes Cereals Snacks Food Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rubber Tire Plastic Recycling Shredder with Factory Price]

300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Various Hose and Output Optional Hose Squeeze Pump Peristaltic.Coill Nail Making Machine/Coil Nail Welding Machine, 175-184[Aeration Wastewater Treatment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar]

Whitaker,J.R.(1963),Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet. Analytical Chemistry35(12), 1950-1953[OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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