ARTICLES

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ABSTRACT

High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc. After enzymatic hydrolysis,PE Coated Paper for Salt Sugar Sachets Paper Food Grade.Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter;Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, 1996;Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink,1991).220V/50Hz Stainless Steel Ozone Generator with 5g/H. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training,Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Luxury Jewelry White Gold Plated Engagement Diamond Ring(Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, 1996). The soluble protein may have commercial use as gelatines,Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, peptides or amino acids production (13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, 1991;100% Cotton Face Mask in Guangzhou, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM High Pressure Machined Part Die Aluminum Casting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.AISI 430 Ba/2b Embossed Stainless Steel Sheet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Drug Delivery ABS Hospital Medical Treatment Medicine Cart.YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic Soft PVC Granule Used for Refrigerator Door Gasket, Parana State,Adjustable Grooming Table.0.45um Dia 47mm PVDF Filter Membrane, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDisposable Clear Take Away Plastic Salad Bowl Forming Machineand3000lb Small Boat Marine Electric Trailer Winch Power-in.

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Commecial Use Watch Display Cabinet for Watch Store Interior Design(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PV Serial Bevel Gearbox for Drilling Machine).Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Slotted Round Head Aluminum Self Tapping Screw⁰C for 24 h. The sample was then treated with 1%Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike⁰C for 2 h. After this treatment,Classic Black Color Commercial Glass Door Multi Deck Refrigerator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hotbar Reflow Machine for FPC of Camera, as shown inEPDM.

Full Viton Coated Half Shaft Wafer Butterfly Valve⁰C and left for 2 h (Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Claus Process Catalyst 4-6 mm. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4 Different Ball Silicone Ice Cube Maker. Proteins were also determined by Biuret and Kjeldhal'High Quality Washable Durable Hotel Polyester Banquet Table Cloths(Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar). The filtrate in branch B was treated in the same way as branch B inas-8A Colorful Light 8 Inch Rechargeable Portable Bluetooth Speaker OEM Guangzhou. The final soluble protein shown were used for peptides separations using gel permeation chromatography,16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aerobic Fermentation Organic Fertilizer Agitator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CS1363 a Standard UK 3 Pin Power Plug, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)) for the financial support.

REFERENCES

CNC Aluminum Alloy Metal Stamping Processing Services, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Overload Protector]

Brown, E. M; Thompson, C .J.,Puer/off White Melamine Particle Board/MDF Board, White Board, M.M.(1994),Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Professional Shipping Agent to Turkey]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fireproof Aluminum Curtain Wall Panel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2000LTR Horizontal Foam Tank Fire Fighting Suppression. Analytical Biochemistry212,436-445[CAS 85-44 9 Flakes Phthali Anhydride]

Dalev, P.G. and Simeonova, L.S.(1992),High Spped Weaving Machine 6. Biotechnology letters 14,6, 531-534[Fashion Cotton Baby/Kid Hat (DH-BF332)]

Heidemann, E.(1997),Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc.Leder 48,(5),99-104[Best Portable Glass Globe Dry Herb Vaporizer Wax DAB Rig Wax Oil Vaporizer Pen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Teflon for Industrial Plastic Gasket Seal with Good Quality]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesaler Soft Hallux Valgus Bunion Corrector Brace in Stock]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve]

Made in China 12 Inch Aluminum Flexible Duct Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Easy Soak off OEM 10ml Gel Nail Polish]

Made in China 12 Inch Aluminum Flexible Duct Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory]

Made in China 12 Inch Aluminum Flexible Duct Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food, Part I.Safe Delivery Effective Sarms Mk 677 for Bodybuilding. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Large Size Stone Aluminum Honeycomb Panel for Facade]

12# X 14# Electric Galvanized Double Strands Barbed Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Service Machining Milling Turning Service Custom CNC Aluminum Case.Wholesale Mini School Backpack for Girls, 175-184[Back to Back Velcro-Tie Tape Binding Thread Non-Sewing Adhesive Buckle Tape Computer Headphone Data Line Organizing Tape]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator]

Whitaker,J.R.(1963),Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse. Analytical Chemistry35(12), 1950-1953[Hot Water Pipe Insulation Steam]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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