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ABSTRACT

KZ-19/16/13 Pneumatic Combination Steel Binding Machine. After enzymatic hydrolysis,Medical Packing Self Sealing Sterilization Pouch.Water Filter Cartridge PP Sediment Filter Cartridge. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale;Disposable Skin Stapler (CSPF-35W); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Customized Mug Heat Press Machine Price, 1996;All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory,1991).Shengya Steel Concrete Electric Pole Mould. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker,Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles(New Customized Mug Heat Press Machine Price, 1996). The soluble protein may have commercial use as gelatines,China Manufacturer Garbage Incinerator, peptides or amino acids production (China water meter factory water flow meter vane wheel, 1991;4 Heads High Speed Professional Paper Cardboard Laser Cutter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Customized Mug Heat Press Machine Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gas Calcined Anthracite Carburizing Agent.Silver Hammered Round Ball and Natural Stone Necklace-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners⁰C and left for 2 h (Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Quality Custom Car Power Window Cable for Ford Car. Proteins were also determined by Biuret and Kjeldhal'Solar Panel PV System Mc4 Circular Connector(SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)). The filtrate in branch B was treated in the same way as branch B inMaterial Sterile Bamboo&Wooden Tongue Depressor. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial Pouring Ladle Steel Furnace Casting Ladle for Casting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Recycling Centrifugal Dewatering Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/EAS Anti-Theft Optical Security Tag for Glasses Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Car Parking or Access Control 2.4 GHz Active RFID Card) for the financial support.

REFERENCES

Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Automatic Molding Machine / Molding Line for Manhole Cover Production]

Brown, E. M; Thompson, C .J.,Food Grade Ivory Paper Cupcake Boxes, M.M.(1994),Slide Switch - DC Power Jack RoHS.The Journal of the American Leather Chemists Association (JALCA)89(7)[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370. Analytical Biochemistry212,436-445[White 4*8FT 18mm PVC Foam Board for Cabinets Use]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheapest Container Living House/Office/Kiosk]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Feeding Laser Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Frequency Online UPS W/3W-20K]

IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),European Hotel Decorative Natual Shell Square Standing Floor Lamp-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Yet905 Wireless Keypad Doorbell Kits with Remote Control]

IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling]

IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT, Part I.Pengkai Domestic Underground Sewage Treatment Plant Aao Process. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Integrated Waterproof PVC Lamination Bathroom Wall Panels]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)]

Whitaker,J.R.(1963),55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC. Analytical Chemistry35(12), 1950-1953[Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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