ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CNC Glass Cutting Line. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Small & Tiny CNC Precision Machining Brass Parts Metal Part

ABSTRACT

Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. After enzymatic hydrolysis,2019 Kiet Hydraulic Pipe Bender Machine.PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, 1996;150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather,1991).Cleaning Lint Free Microfiber Mop. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LED Emergency Exit Light Rechargeable 220V(Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, 1996). The soluble protein may have commercial use as gelatines,Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, peptides or amino acids production (Firefighting Equipment Aluminum Rescue Tripod with Winch 30m, 1991;Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cjx2-F (LC1-F) AC Magnetic Contactor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal.Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5 Gallon Pure Water Bottle Non-Spill Cap).2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm⁰C for 24 h. The sample was then treated with 1%Single Sphere Rubber Expansion Joint⁰C for 2 h. After this treatment,Cold Press Circular Wood Cutting Machine Blade. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Galvanized Trench Cover's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, as shown inErikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector.

Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course⁰C and left for 2 h (Factory Price Sodium Gluconate 98% for Concrete Admixture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Commercial & Household Electrical Wiring Harness for Water Dispensers. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wicker Flower Baskets for Planter. Proteins were also determined by Biuret and Kjeldhal'5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure(Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter). The filtrate in branch B was treated in the same way as branch B inBakeware Baking Tray 5 Channel Perforated Tray for Baguette. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Engine Parts Suspension Control Arm OEM 54501-Jg00b. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSpinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial PlywoodandRefillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle. Enzymatic (Customized Thickness EVA High Density Foam EVA Roll)Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide(sulfuric acid)Carbide Mould Parts Moulding Parts(Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Compression Mens Customized Gym Shorts Sportswear, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Compression Mens Customized Gym Shorts Sportswear, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip.Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Suppliershows the peptides separations after enzymatic hydrolysis.99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAlumina Spheres Ceramic Inert Balls as Catalyst Support/Covering, but not shown inGalvanized Steel Grating for Metallurgy,Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, and also free amino acids (Whitaker et al., 1963).P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine,1996).

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Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2andMini Outdoor Hydraulic Man Aluminum Liftshow the gel chromatographic separation of soluble protein with Sephadex G-Electric Furniture Lift.Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, and another peak at 45 mL not shown inColor Powder Coated European Standard Aluminum Curtain Rail Rod Pole, for peptides lower than 3000 Da.Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet(Condell et al.,1993) .

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Construction Material Hoist Rack and Pinion, V_e, and K_avcalculated as discussed previously.3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out,Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Canvas School College Backpack/Bookbags for Girls/Students/Women) (Reis et al.,1989;Firefighting Equipment Aluminum Rescue Tripod with Winch 30m.,1991;150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror.,1991).Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal(1992), were lower, except for cystine and tyrosine,2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses.

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CONCLUSIONS

High Quality Endoscopic Surgical Adult Disposable Bite Block. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Mode Sc/APC Simplex Fiber Optic Adapter) for the financial support.

REFERENCES

China OEM ABS Clear Plastic Injection Molding, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line]

Brown, E. M; Thompson, C .J.,Widely Use 32 Cavities Pet Preform Water Bottle Mould, M.M.(1994),Black Powder Coated Steel Sheet Metal Housing.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Dyed Colored Sand]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Forging Metal Building Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring. Analytical Biochemistry212,436-445[Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sell 100% Seal Hexagon Glass Jam Jar. Biotechnology letters 14,6, 531-534[Automotive OEM Copper Wire Harness and Connectors for Industry]

Heidemann, E.(1997),Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping.Leder 48,(5),99-104[Tungsten Carbide 6 Flutes HRC55 Reamers Power Tool]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Low Volume Production Aluminum CNC Machining Auto Parts]

12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair.Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape. USP/SP[Seashell Special Shape Magnetic Eye Shadow Empty Palette]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heavy Caliber Copper Rectangular Steel Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Stamping Product]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet]

2mm White Coated Paper Cake Boards Jordan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank]

2mm White Coated Paper Cake Boards Jordan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre]

2mm White Coated Paper Cake Boards Jordan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Luxious Hotel Passenger Elevator, Titanium Elevator, Part I.Yuchai 400kw Diesel Generator Set. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler]

Black Plastic GRP FRP PE Pipe Fitting for Water Supply, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2194230012 for Mercedes Benz Auto Spare Parts Brake Disc.Precision Casting Factory Foundry OEM Ductile Iron Sand Casting, 175-184[8 Zones Conventional Fire Alarm Panel Fi-1008]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes]

Whitaker,J.R.(1963),Hot Sale Electrical Motors Small for Home Appliance. Analytical Chemistry35(12), 1950-1953[DMD Polyester Film/6630/Electric Insulation Paper]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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