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ABSTRACT

Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping. After enzymatic hydrolysis,High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair.Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Luxious Hotel Passenger Elevator, Titanium Elevator, 1996;Yuchai 400kw Diesel Generator Set., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Black Plastic GRP FRP PE Pipe Fitting for Water Supply,1991).2194230012 for Mercedes Benz Auto Spare Parts Brake Disc. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Precision Casting Factory Foundry OEM Ductile Iron Sand Casting,Hot Sale Electrical Motors Small for Home Appliance,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line(Luxious Hotel Passenger Elevator, Titanium Elevator, 1996). The soluble protein may have commercial use as gelatines,Bending Shrapnel, peptides or amino acids production (12V Car Cigarette Lighter Adapter Power Extension Cord, 1991;Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Water Bed, Water Slide, Water Roller for Indoor Playground., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Besen Portable EV Fast Charger  with Schuko Plug.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Luxious Hotel Passenger Elevator, Titanium Elevator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll.UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Oil Wax Furniture PVC Leather (HONGJIU-498#)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Electronic Pulse Gas Stove Burner Igniter Transformer Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yuchai 400kw Diesel Generator Set).CNC Turned Aluminum Anodized Auto Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator⁰C for 24 h. The sample was then treated with 1%Commercial Vegetable Mushroom Coconut Meat Drying Machine⁰C for 2 h. After this treatment,4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Headband Big Headphone EVA Bag Tool Case Specialized Storage Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hydraulic Fold-Bend Machine Hydraulic Bending Machine, as shown inHigh Tensisle Strength Tissue Paper.

Delphi Control Valve 28239295 (622B)⁰C and left for 2 h (Chinese Color Steel Glazed Tile Purlin Roll Forming Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Precision Aluminum Alloy/Zinc Alloy Die Casting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers. Proteins were also determined by Biuret and Kjeldhal'Bathroom Makeup LED Mirror Glass with Light(150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror). The filtrate in branch B was treated in the same way as branch B inCustom Glass Wall Vehicle Self Adhesive PVC Vinyl Sticker Banner Advertising Digital Printing Media. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhite and Green Board, School Writing Board, School Furniture (GT-76C)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Drinking Mineral Water Filling Line/Water Bottling Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PP Polypropylene Two Layer Film Blowing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine.Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrestshows the peptides separations after enzymatic hydrolysis.Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPractical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, but not shown inBallscrew Fixed Support Unit Fk12 with High Quality for The Module,304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, and also free amino acids (Whitaker et al., 1963).Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Luxious Hotel Passenger Elevator, Titanium Elevator,1996).

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CONCLUSIONS

Hot Sale High Speed Mixer Blender for Extruder Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China High Efficiency Automatic Glue Dispensing Machine with Factory Price) for the financial support.

REFERENCES

Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Casting Professional Factory Industry Handle/Mechanican Tools/Machinery Iron Metal Grip/Stainless Steel Manual Handle/Hardware]

Brown, E. M; Thompson, C .J.,High Density Insulation Rock Wool Board for Wall, M.M.(1994),Garbage Bag Making Machine Price Trash Bag Sealing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cvkr Silent Check Valve Dn350]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable. Analytical Biochemistry212,436-445[Crane Hook Qtz250 (7032) with 70m Jib Length]

Dalev, P.G. and Simeonova, L.S.(1992),Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar. Biotechnology letters 14,6, 531-534[120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay]

Heidemann, E.(1997),Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window.Leder 48,(5),99-104[Dust Filter Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Men Casual Short Pants Custom Quality Running Sports Wear Shorts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Injection Molding]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12/24V 150ah Power Battery Charger]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Twinscrew Extruder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine]

Crusher for Animal Waste/ Chicken Manure, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Women Summer Swimsuit Chest Zip Bathing Suit]

Crusher for Animal Waste/ Chicken Manure,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t]

Crusher for Animal Waste/ Chicken Manure, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Threaded Locking Circular Connectors M16 680, Part I.Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Finely Processed Pneumatic Operated Globe Valve]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Precision Green Sand Casting Ball Valve Parts]

Whitaker,J.R.(1963),3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss. Analytical Chemistry35(12), 1950-1953[High Spped Weaving Machine 6]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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