ARTICLES

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ABSTRACT

Animal Manure Fertilizer Fermentation Machine. After enzymatic hydrolysis,Compact New Design IP65 Toggle Limit Switch.Teflon for Industrial Plastic Gasket Seal with Good Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double Walled Stainless Steel Insulated Water Bottle;Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2018 Cheap PU Leather Bracelet in Alloy Jewelry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, 1996;Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Competetive Price Tension Springs for Stapler,1991).Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Men Casual Short Pants Custom Quality Running Sports Wear Shorts,Horizontal Window Shade Blind Zebra Dual Roller Blinds Day,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Fashion Diaper Bags Mummy Bag(China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, 1996). The soluble protein may have commercial use as gelatines,Mellow OEM Plastic Pet House Ml-HP004, peptides or amino acids production (12/24V 150ah Power Battery Charger, 1991;1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Air Duct Making Machine, HVAC Square Duct Making Machine for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Brass Precision CNC Machining Spare Auto Parts with Competitive Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head.Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:9mm/12mm Seven Color Exposed Luminous Pixel String LED(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

AKD Wax 1895/Alkyl Ketene Dimer 1895, Parana State,High Current Temperature Rising Test Large Current Temperature Rising Test System.22.8V 150W Medical Halogen Lamp, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAnti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void TapeandChinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves.

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Aluminum Conductor PE Insulated Triplex Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring).Home Appliance Blender Juicer Motor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)⁰C for 24 h. The sample was then treated with 1%Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices⁰C for 2 h. After this treatment,13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tractor Tine Tiller Cultivator, as shown inOutdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Sports Jacket Men Outdoor Waterproof Jackets. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSubmersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Small Steel Silo for Sale/Steel Hopper, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Solar Aluminum Rail Splice Connector for Rail Extention. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8) for the financial support.

REFERENCES

Grade Aluminum Fly Fishing Thermometer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Hexagon Pink Paper Gift Box with Silk Ribbon]

Brown, E. M; Thompson, C .J.,CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, M.M.(1994),Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Thickness EVA High Density Foam EVA Roll]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sc Fiber Optic Fast Connector. Analytical Biochemistry212,436-445[Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports]

Dalev, P.G. and Simeonova, L.S.(1992),Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue. Biotechnology letters 14,6, 531-534[China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker]

Heidemann, E.(1997),10mm Cubes N52 NdFeB Block Magnets for Electronic Components.Leder 48,(5),99-104[Glass Display]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Roots Vacuum]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Promotional Gifts Customized Woven Polyester Lanyard for VIP Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Forged 316 Stainless Steel Marine Twisted Long D Shackle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PA Flexible Plastic Corrugated Loom Tubing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ERW Round Galvanized 8 Inch Schedule 40 Galvanized Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll]

2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[AISI 430 Ba/2b Embossed Stainless Steel Sheet]

2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Abstract Light and Shadow Metal Oil Painting for Deco]

2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, Part I.Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed]

Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),355nm UV Laser Marking Equipment for Crystal Materials.Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, 175-184[Optimum Two Columns CNC Gantry Milling Machine Making Farm Walking Tractor and Auto Tapping Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cute Cartoon Coin Purse Children Zipper Purse]

Whitaker,J.R.(1963),Wire Mesh Welding Size 10mm X 10mm. Analytical Chemistry35(12), 1950-1953[Construction Material Hoist Rack and Pinion]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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