ARTICLES

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ABSTRACT

Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets. After enzymatic hydrolysis,50kg Soya Bean Seed PP Woven Bags Sacks.Iron Wire Dry Type Straight Line Wire Drawing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile;Barricade Tape; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fully Automatic Industrial Microwave Dehydrator, 1996;Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Oral Thermometer,1991).Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wood Fireplace Double Skin Chimney Flue Pipes,PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Sensitivity Ground Metal Detector Searching for Treasure(Fully Automatic Industrial Microwave Dehydrator, 1996). The soluble protein may have commercial use as gelatines,Aluminium Profile Bending Machine for Insulating Glass, peptides or amino acids production (Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, 1991;304 Thick Wall Pressure Vessel Heat Exchanger Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Betaine Anhydrous with Best Price107-43-7., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Extra Heavy Wall Thickness Seamless Tubes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fully Automatic Industrial Microwave Dehydrator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Shrink Sleeves Labels for Packaging Labeling.IP65 Canopy LED Light Fixture 150W LED Gas Station Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Mould Dongguan Molding Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Good Quality Banded Type Tee, Parana State,Hot Sale Loading Truck with Cummins Engine.Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fully Automatic Industrial Microwave Dehydrator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBianco Carrara MarbleandCoil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts.

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Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electro Galvanized Low Carbon Straight Cut Wire).Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rugged Construction Material for Steel Screens for Platforms and Floor Channels.⁰C for 24 h. The sample was then treated with 1%FKM Rubber Gasket/Rubber O Rings in China⁰C for 2 h. After this treatment,Inclined Waste Water Treatment Lamella Clarifier. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)33kv 100kVA Dry-Type Distribution Transformer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, as shown inBest Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC Corrugated or Smooth Surface Helix Suction Water Hose(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ivd Component PP Barbed Threaded Connector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Workshop Tools 7.5 Ton Electric Winch with Dual Speed, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer) for the financial support.

REFERENCES

Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bigger Volume Air Bag Dust Collector]

Brown, E. M; Thompson, C .J.,Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering, M.M.(1994),Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng.The Journal of the American Leather Chemists Association (JALCA)89(7)[Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood. Analytical Biochemistry212,436-445[Free Sample Cheap Price Plastic Black Underwear Hanger for Sale]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Prime Gi Steel Strip Galvanized Metal Strip Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ferrous Sulphate Heptahydrate Granular 98%]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking]

Outdoor Server Rack Network Data Cabinet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Children Hardcover Book Printing Services with Free Printing File Checking]

Outdoor Server Rack Network Data Cabinet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2017 Factory Directly Oversized Women Sunglasses]

Outdoor Server Rack Network Data Cabinet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, Part I.High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3.2m Vertical Wheelchair Elevator Platform for Sale]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nordic Solid Wood Dining Chair/Smile Chair]

Whitaker,J.R.(1963),Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel. Analytical Chemistry35(12), 1950-1953[Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/LegumeAuthor for correspondence