ARTICLES

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ABSTRACT

100% Cotton Face Mask in Guangzhou. After enzymatic hydrolysis,OEM High Pressure Machined Part Die Aluminum Casting.AISI 430 Ba/2b Embossed Stainless Steel Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, 1996;Colorful Electric English Funny Music Story Module Printing Book., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine,1991).IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tilt-and-Turn-with-Hidden-Hinge,Automatic Soft PVC Granule Used for Refrigerator Door Gasket,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,0.45um Dia 47mm PVDF Filter Membrane(Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, 1996). The soluble protein may have commercial use as gelatines,Commecial Use Watch Display Cabinet for Watch Store Interior Design, peptides or amino acids production (Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, 1991;Slotted Round Head Aluminum Self Tapping Screw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Classic Black Color Commercial Glass Door Multi Deck Refrigerator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hotbar Reflow Machine for FPC of Camera.Full Viton Coated Half Shaft Wafer Butterfly Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Claus Process Catalyst 4-6 mm(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Aerobic Fermentation Organic Fertilizer Agitator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts).Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CS1363 a Standard UK 3 Pin Power Plug⁰C for 24 h. The sample was then treated with 1%Insulation Anti Earthquake Exterior Wall Foam Board⁰C for 2 h. After this treatment,Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Easy Soak off OEM 10ml Gel Nail Polish's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, as shown in3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording.

Recyclable PU Leather Jewelry Pouch⁰C and left for 2 h (Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Activated Alumina Used for Catalyst Carrier in Chemical Industry. Proteins were also determined by Biuret and Kjeldhal'High Quality 45W 50W Commercial Recessed COB LED Downlight(304 Thick Wall Pressure Vessel Heat Exchanger Pipe). The filtrate in branch B was treated in the same way as branch B inGSM Series Antenna Customized Connector Flat GSM Antenna. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Auto Corrugated Carton Box Paperboard Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Waterproof BNC Connector for Industry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Indoor Inkjet Media Material, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female) for the financial support.

REFERENCES

Wholesale Mini School Backpack for Girls, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Em 4100 Tk4100 RFID125kHz ID PVC Proximity Smart Card]

Brown, E. M; Thompson, C .J.,Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, M.M.(1994),Super Potassium Humate Shiny Flakes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ballscrew Fixed Support Unit Fk12 with High Quality for The Module]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4K Giant Whiteboard Interactive Panel for University Whiteboard. Analytical Biochemistry212,436-445[Rectangular Lunch Tin Box with Handle]

Dalev, P.G. and Simeonova, L.S.(1992),Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25. Biotechnology letters 14,6, 531-534[Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)]

Heidemann, E.(1997),Electric Massage Bed Pedicure Chair with Double Dust Collector.Leder 48,(5),99-104[Chain Drive Rolling Door Opener]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe]

Luxury Jewelry White Gold Plated Engagement Diamond Ring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle.Adjustable Solar Mounting Kits (GD1059). USP/SP[Weak Daylight Recharge All in One Solar Street Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ SBPP]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bgf-800 BOPP Dry Laminating Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Piggery Farm Pig/Sow Gestation Equipment From China Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Spraying Machine For Bed Side Table Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Inclined Waste Water Treatment Lamella Clarifier]

Test-630 Automatic Protective Relay Tester, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gas Calcined Anthracite Carburizing Agent]

Test-630 Automatic Protective Relay Tester,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure]

Test-630 Automatic Protective Relay Tester, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tpdl Belt Press Sludge Equipment with Slaughtering Machine, Part I.3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cvkr Silent Check Valve Dn350]

The Most Demanded Products Fluorescence Microscope Camera, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Low Price Promotion Soda Ash Light to Papermaking.Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle, 175-184[5mm 216 PCS Magnets Sphere Magnet Ball Magnet Cube Neo Cube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Crane Hook Qtz250 (7032) with 70m Jib Length]

Whitaker,J.R.(1963),HOWO 420HP Rhd Tractor Truck Head. Analytical Chemistry35(12), 1950-1953[120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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