ARTICLES

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^IICNPq/RHAE/biotechnology-Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture

ABSTRACT

China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). After enzymatic hydrolysis,Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor.Rubber Tire Plastic Recycling Shredder with Factory Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft;Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Mens Cotton Reflective Tape Work Pants, 1996;Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Coil with AA3003 AA3105 H14 H16 H18,1991).High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DC Stand Fan Motor PCBA Card 24V,Economic Brwon Kraft Slip Sheet Tray and Pallet Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel 304 Wire Rope Fork Terminal(Industrial Mens Cotton Reflective Tape Work Pants, 1996). The soluble protein may have commercial use as gelatines,Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag, peptides or amino acids production (Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, 1991;Power Tools Lithium Battery Cordless Drill (GBK2-66108TS), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CAS 85-44 9 Flakes Phthali Anhydride., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Mens Cotton Reflective Tape Work Pants, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Good Design Custom Made Cheap Recycle Paper Bags.Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Non-Ionic Polyacrylamide Flocculant(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Blue Silica Gel with Indicating(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike).Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock⁰C for 24 h. The sample was then treated with 1%Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)⁰C for 2 h. After this treatment,Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Precision Green Sand Casting Ball Valve Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, as shown inDocument Storage Durable Metal Wire Mesh Decking for Pallet Rack.

Class 300 1/2 Stainless Steel Forging Type Check Valve⁰C and left for 2 h (Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CAD Cam Dental Machine 3D Dental Model Printer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,900kg Pneumatic Motorcycle Lift. Proteins were also determined by Biuret and Kjeldhal'Custom Power Extension Cord AC Cable Plug for Office Equipment(China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board). The filtrate in branch B was treated in the same way as branch B in200W CO2 Laser Cutting Machine Cooling System (CW-5300). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5 Liter Packed Medical Ultrasound Gel with Ce and ISO. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Vmc Machine Auto Parts Processing CNC Vertical Machining Centre(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Etched Stainless Steel Titanium Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Small Brown Glass Bottles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks) for the financial support.

REFERENCES

Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Honkon Beauty SPA Skin Care and Acne Removal Mesogun Injector Medical Machine]

Brown, E. M; Thompson, C .J.,Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe, M.M.(1994),RF China Professional Manufacturer Direct Way Manual Ball Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[SGS 40L Lab Use Chocolate Refiner Conching Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Carbon Stee Semless Pipe (ASTM A106 Gr B)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected. Analytical Biochemistry212,436-445[Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Braided PTFE Hose R14 Standard Pipe. Biotechnology letters 14,6, 531-534[Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine]

Heidemann, E.(1997),SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser.Leder 48,(5),99-104[Antiskid Bike Pedal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Direct From China Education Toy Doctor Kit Set Toy]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price]

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500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flat Head Six-Lobe with Gasket Self Tapping Screws]

500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1200X1000X810 Strong Collapsible Plastic Containers for Storage, Part I.Fast Install Polyurethane Decorative Wall Panel for Homes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit]

Whitaker,J.R.(1963),Cracking Acidification Clay Stabilizer Pale Yellow Liquid. Analytical Chemistry35(12), 1950-1953[Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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