ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Competetive Price Tension Springs for Stapler. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine

ABSTRACT

Men Casual Short Pants Custom Quality Running Sports Wear Shorts. After enzymatic hydrolysis,Horizontal Window Shade Blind Zebra Dual Roller Blinds Day.New Fashion Diaper Bags Mummy Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mellow OEM Plastic Pet House Ml-HP004;12/24V 150ah Power Battery Charger; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, 1996;China Brass Precision CNC Machining Spare Auto Parts with Competitive Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box,1991).044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t,9mm/12mm Seven Color Exposed Luminous Pixel String LED,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Construction Scaffolding Steel Galvanized Acrow Prop(Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, 1996). The soluble protein may have commercial use as gelatines,3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, peptides or amino acids production (Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8, 1991;Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;AKD Wax 1895/Alkyl Ketene Dimer 1895., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.22.8V 150W Medical Halogen Lamp.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Conductor PE Insulated Triplex Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Home Appliance Blender Juicer Motor.Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LSR Injection Mold Tooling for Medical Nipple(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)).Nail Stamping Plates Custom Metal Part Sheet Metal Stamping(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Sports Jacket Men Outdoor Waterproof Jackets⁰C for 24 h. The sample was then treated with 1%Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller⁰C for 2 h. After this treatment,Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Small Steel Silo for Sale/Steel Hopper, as shown inNewest Adjustable Decline Bench Flat Weight Bench for Wholesales.

Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality⁰C and left for 2 h (Amplifier Tube Glass Vacuum Tube (833C)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Smart Big Hydroponic Indoor Home Garden Planter. Proteins were also determined by Biuret and Kjeldhal'China Quality Heavy Duty Globe Control Valve(Large Diameter Aluminum Pipe 3004). The filtrate in branch B was treated in the same way as branch B inCabinet Accessories Cabinet Three-Layer Shoes Rack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Suppliers Laser Marking Equipment of Fiber Laser. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBonfiglioli Ta Series Helical Gearing Gearbox(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Large Stock Aerators for Shrimp Farms and Fish Pond, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

12# X 14# Electric Galvanized Double Strands Barbed Wireand750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.. Enzymatic (Large Diameter Aluminum Pipe 3004)Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory(sulfuric acid)OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder(Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Integrated Waterproof PVC Lamination Bathroom Wall Panels, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Integrated Waterproof PVC Lamination Bathroom Wall Panels, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tractor Tine Tiller Cultivator) for the financial support.

REFERENCES

Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chip Board Blocks]

Brown, E. M; Thompson, C .J.,Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, M.M.(1994),Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight. Analytical Biochemistry212,436-445[Single Block Vertical Wire Drawing Machine for Screw and Bolt Making]

Dalev, P.G. and Simeonova, L.S.(1992),355nm UV Laser Marking Equipment for Crystal Materials. Biotechnology letters 14,6, 531-534[Better Quality White Coated Fbb Paper Ivory Board]

Heidemann, E.(1997),Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB.Leder 48,(5),99-104[2019 New Model Grain Dryer Machine for 30t/Batch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4lz-4.5 Small Tank Kubota Similar Rice Harvester]

Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wire Mesh Welding Size 10mm X 10mm.P4.75 Indoor Single Dual Color Advertising LED Display Billboard. USP/SP[Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Rigging Screw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Pipe Tube Curving Machine with Super Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mmjp Series Rice Grading Machine Rice Grader]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Premium quality for Potassium Humate powder]

Plug-in Assembly Type Straight Through Joint and Insulation Joint, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hydraulic Cutter Bread Machine (ZMF-20Y)]

Plug-in Assembly Type Straight Through Joint and Insulation Joint,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100%Rayon Red Satin Dyeing Fabric for Women Dress]

Plug-in Assembly Type Straight Through Joint and Insulation Joint, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, Part I.Quilting Machine for Muti- Needle Mattress Quilting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Water Bed]

Single-Screw Plastic Extruders Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube.High Quanlity Colorful Aluminum Spacer/Standoff, 175-184[Hydraulic Change-Over Valve 6/2 Paths 12 Volt 3/8 50 L / Min]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin]

Whitaker,J.R.(1963),5g OPP Silica Gel Desiccant. Analytical Chemistry35(12), 1950-1953[Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pre Painted Steel Coil and SheetAuthor for correspondence