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ABSTRACT

Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain. After enzymatic hydrolysis,Waterproof LED Flash Shoe Light Crystal Epoxy Resin.Custom Shopping Paper Bag for Clothing Package. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Qingdao Us Type Load Binder Lever Chain Tensioner;Stainless Steel Elbow SS304 SS316L; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor 15kv Pole Mounted Automatic Circuit Recloser, 1996;Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (14 Heads Double Door Multihead Weigher,1991).Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,White Marble, Carrara White, Bianco Carrara Marble for Sale,Upper Limbs Machine/Spare Parts for Fitness Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cvkr Silent Check Valve Dn350(Outdoor 15kv Pole Mounted Automatic Circuit Recloser, 1996). The soluble protein may have commercial use as gelatines,Best Shatterproof Gold Rim Wine Glasses, peptides or amino acids production (10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting, 1991;High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor 15kv Pole Mounted Automatic Circuit Recloser, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chinese Color Steel Glazed Tile Purlin Roll Forming Machine.Industrial Sausage Meat Smoker Oven/Smokerhouse Oven-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Men′s High Quality Mercerised Cotton Business Dress Socks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S), Parana State,1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector.2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Outdoor 15kv Pole Mounted Automatic Circuit Recloser, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePiggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry FarmandAfraic Market Mobile Food Vending Cart.

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Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line).E6000 Handheld Three Phase Power Quality and Energy Analyzer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Qg Jg Fg Series Air Dryer Equipment Factory⁰C for 24 h. The sample was then treated with 1%Motor Bearing 6014 2RS Deep Groove Ball Bearing⁰C for 2 h. After this treatment,PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pet Short Fiber Non Woven Geotextile for Landfill Filtration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pre Painted Steel Coil and Sheet, as shown inCNC Machining Mechanical Parts Fabrication Service.

Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd⁰C and left for 2 h (Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8). Proteins were also determined by Biuret and Kjeldhal'Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion(23m 4 R Arms 2017 New Design Concrete Placing Boom). The filtrate in branch B was treated in the same way as branch B inCustomize Powder Coating Steel Stamping Terminal Cable Equipment Mounting Bracket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Maleic Anhydride CAS 108-31-6.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic HDPE Pipe Extrusion Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avJaguar Jb-390 Automatic PCB Loader and Unloader(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Factory Organic Ethyl Ascorbic Acid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Price Sodium Gluconate 98% for Concrete Admixture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic EnclosureandPneumatic Cut off Ball Valve with Flange Thread Weld End. Enzymatic (Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine)Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar(sulfuric acid)Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor(Outdoor 15kv Pole Mounted Automatic Circuit Recloser, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Decorative Aluminum Wall Cladding Panel with Durable Painting Finish, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Decorative Aluminum Wall Cladding Panel with Durable Painting Finish, flow rate of 0.1 mL/min., and l = 620 nm.

Steel Grating for Industrial Platform.Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trimshows the peptides separations after enzymatic hydrolysis.50Hz/60Hz Low Frequency Home Power Inverter 5000W(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSupply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V, but not shown inFull Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials,ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line, and also free amino acids (Whitaker et al., 1963).Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Outdoor 15kv Pole Mounted Automatic Circuit Recloser,1996).

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CONCLUSIONS

From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine) for the financial support.

REFERENCES

Mini Double Hole ABS Plastic CNC Machined Parts Importer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Laser For Cutting Sheet Metal Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,KZ-19/16/13 Pneumatic Combination Steel Binding Machine, M.M.(1994),Medical Packing Self Sealing Sterilization Pouch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cleaning Lint Free Microfiber Mop]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Water Filter Cartridge PP Sediment Filter Cartridge. Analytical Biochemistry212,436-445[China Factory Queen Mattress with Visco Memory Foam and Latex]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale. Biotechnology letters 14,6, 531-534[Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box]

Heidemann, E.(1997),Disposable Skin Stapler (CSPF-35W).Leder 48,(5),99-104[Fancy Rubber Gloves]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors]

Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827).New Customized Mug Heat Press Machine Price. USP/SP[220V/50Hz Stainless Steel Ozone Generator with 5g/H]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solar Panel PV System Mc4 Circular Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Silver Hammered Round Ball and Natural Stone Necklace]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laptop Backpack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Right Angle Worm Gearbox for Food and Beverage and Packaging Machines]

Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Shengya Steel Concrete Electric Pole Mould-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable]

Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED Emergency Exit Light Rechargeable 220V]

Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker, Part I.Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting]

Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Manufacturer Garbage Incinerator.China water meter factory water flow meter vane wheel, 175-184[Galvanized Tube Steel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Aluminum Alloy Metal Stamping Processing Services]

Whitaker,J.R.(1963),4 Heads High Speed Professional Paper Cardboard Laser Cutter. Analytical Chemistry35(12), 1950-1953[Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

14 Gauge Twist Galvanized Black Annealed Steel WireAuthor for correspondence