ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,OEM Round Stainless Steel Fruit Plate Tray

ABSTRACT

Factory Supply Industry Grade Sodium Gluconate. After enzymatic hydrolysis,Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set.En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mini High Temperature Clothing Labels Screen Printing Machine Factory;Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, 1996;Conventional Best-Selling Household Bamboo Craft Chopsticks., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange,1991).Ce Marked Medical Syringe Pump (WP08N). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty,Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera(Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, 1996). The soluble protein may have commercial use as gelatines,Electro Galvanized Low Carbon Straight Cut Wire, peptides or amino acids production (Geotextile for Roofs, Civil Applications. Railway Work, 1991;Pneumatic Shuttle Valve Flange Ends, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stx825 Dual 15 Powerful PA Speaker, Speaker Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ce Approval Biscuit Trayless Packing Machine (MZ-250B).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible.Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Superior Quality Soya Protein Food Production Machine Line).Supply 20-110mm PP Reducing Tee for Pipe Systems(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5 Gallon Pure Water Bottle Non-Spill Cap⁰C for 24 h. The sample was then treated with 1%Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks⁰C for 2 h. After this treatment,7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Solid Carbide Single Flute End Mills Oblique Angle Cutting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies, as shown inWaterproof LED Flash Shoe Light Crystal Epoxy Resin.

ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat⁰C and left for 2 h (Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Small Capacity Automatic Cup Filling Machine. Proteins were also determined by Biuret and Kjeldhal'Seasoned Cast Iron South Africa Three Legged Potjie Pot(3mm Indoor Wall Cladding Decoration Aluminum Composite Material). The filtrate in branch B was treated in the same way as branch B inHydrauic Four Column Machine Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2019 New Release Doypack Sachet Powder Packing Machine Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Style Custom Logo Makeup Multi Colored Eyeshadow Palette, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2mm White Coated Paper Cake Boards Jordanand20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale. Enzymatic (30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu)High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume(sulfuric acid)Daytai 1550nm External Modulation Optical Transmitter 1550 -50km(Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Promotion Indian Market Underwear Adhesive Glue.Pipe Thermostat with Sensor Ce Certificateshows the peptides separations after enzymatic hydrolysis.700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlastic Mould Dongguan Molding Factory, but not shown in4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass,Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files, and also free amino acids (Whitaker et al., 1963).New Industrial Wheeled Wooden Shoe Display Fixtures for Shop. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts,1996).

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Plastic Injection Mould with S136 Mould Steel Plastic Mould FactoryandSmall & Tiny CNC Precision Machining Brass Parts Metal Partshow the gel chromatographic separation of soluble protein with Sephadex G-Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness.Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, and another peak at 45 mL not shown inLED Indoor Light Linear Light Aluminium LED Profile, for peptides lower than 3000 Da.Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light(Condell et al.,1993) .

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CONCLUSIONS

Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (White Pattern Acrylic Sheet for Decoration Produced by Senpu) for the financial support.

REFERENCES

Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1 Inch Lcd Module]

Brown, E. M; Thompson, C .J.,Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, M.M.(1994),Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory. Analytical Biochemistry212,436-445[Fully Automatic Digital Pid Temperature Controller]

Dalev, P.G. and Simeonova, L.S.(1992),Masturbators Adult Sex Toys Japanese Love Doll. Biotechnology letters 14,6, 531-534[Competetive Price Tension Springs for Stapler]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot-Press Small Continuous Diamond Saw Blade Cutting Disc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electric Shock Butt Plug]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Drinking Mineral Water Filling Line/Water Bottling Line]

IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Powder Spray Booth for Metal Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Bianco Carrara Marble]

IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts]

IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Small Tin Box for Condoms, Part I.Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale]

Petrochemical Loading Platform Folding Stairs, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),64mm Double Braid UHMWPE Tugboat Mooring Rope.Rubber Soles Vulcanizing Machine/ Press Machine, 175-184[10000 Cycles 6.35mm Audio SMD Stereo Phone Jacks Earphone Jack]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electro Galvanized Low Carbon Straight Cut Wire]

Whitaker,J.R.(1963),Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable. Analytical Chemistry35(12), 1950-1953[Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wholesale Professional Indoor&Outdoor Trampoline ParkAuthor for correspondence