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ABSTRACT

Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel. After enzymatic hydrolysis,Prime Gi Steel Strip Galvanized Metal Strip Price.Fireproof Aluminum Curtain Wall Panel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Adjustable Grooming Table, 1996;High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PE Coated Paper for Salt Sugar Sachets Paper Food Grade,1991).Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter,Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,15K High Frequency Online UPS UPS RS232/USB/Snmp Optional(Adjustable Grooming Table, 1996). The soluble protein may have commercial use as gelatines,Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, peptides or amino acids production (Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate, 1991;Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;220V/50Hz Stainless Steel Ozone Generator with 5g/H., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Adjustable Grooming Table, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxury Jewelry White Gold Plated Engagement Diamond Ring.Free Sample Cheap Price Plastic Black Underwear Hanger for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Drug Delivery ABS Hospital Medical Treatment Medicine Cart, Parana State,M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Adjustable Grooming Table, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTwo - Way Double Row Thrust Tapered Roller BearingsandChina Superior Quality Soya Protein Food Production Machine Line.

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Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses).Colorful Electric English Funny Music Story Module Printing Book(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine⁰C for 24 h. The sample was then treated with 1%IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)⁰C for 2 h. After this treatment,Tilt-and-Turn-with-Hidden-Hinge. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Soft PVC Granule Used for Refrigerator Door Gasket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)0.45um Dia 47mm PVDF Filter Membrane, as shown in3mm Indoor Wall Cladding Decoration Aluminum Composite Material.

Commecial Use Watch Display Cabinet for Watch Store Interior Design⁰C and left for 2 h (2mm White Coated Paper Cake Boards Jordan). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Slotted Round Head Aluminum Self Tapping Screw. Proteins were also determined by Biuret and Kjeldhal'Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike(Pipe Thermostat with Sensor Ce Certificate). The filtrate in branch B was treated in the same way as branch B inBus/RV Shelter (JIT-1865). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Classic Black Color Commercial Glass Door Multi Deck Refrigerator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHotbar Reflow Machine for FPC of Camera(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Claus Process Catalyst 4-6 mm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4 Different Ball Silicone Ice Cube Maker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Washable Durable Hotel Polyester Banquet Table Cloths, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (AISI 430 Ba/2b Embossed Stainless Steel Sheet) for the financial support.

REFERENCES

Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[MPO Optical Fiber Adapter Adaptor]

Brown, E. M; Thompson, C .J.,High Speed Auto Corrugated Carton Box Paperboard Production Line, M.M.(1994),Custom Waterproof BNC Connector for Industry.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fully Automatic Digital Pid Temperature Controller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Competetive Price Tension Springs for Stapler]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Indoor Inkjet Media Material. Analytical Biochemistry212,436-445[Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage]

Dalev, P.G. and Simeonova, L.S.(1992),Steel Jacket Steam Insulation Pipeline Production Famouse in China. Biotechnology letters 14,6, 531-534[Vibrating Condom Ring and Cock Ring Supplier]

Heidemann, E.(1997),Solid Steel Hotel Safe (G-42BD classics) for Hotel.Leder 48,(5),99-104[Cement Foaming Agent Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control]

LC Multistage Pump Mechanical Seal (KLLC), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c.CNC Aluminum Alloy Metal Stamping Processing Services. USP/SP[Hot-Press Small Continuous Diamond Saw Blade Cutting Disc]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Drinking Mineral Water Filling Line/Water Bottling Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Puer/off White Melamine Particle Board/MDF Board, White Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Bianco Carrara Marble]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Level Sensor For Solids]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts]

Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2000LTR Horizontal Foam Tank Fire Fighting Suppression-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale]

Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electro Galvanized Low Carbon Straight Cut Wire]

Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Spped Weaving Machine 6, Part I.Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture]

Small Poultry Pig Feed Farm Steel Silo for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine.P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, 175-184[Metal]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Professional Indoor&Outdoor Trampoline Park]

Whitaker,J.R.(1963),Made in China 12 Inch Aluminum Flexible Duct Pipe. Analytical Chemistry35(12), 1950-1953[Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

IronAuthor for correspondence