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ABSTRACT

Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml. After enzymatic hydrolysis,Pickled Cucumber Production Line/Making Machine.Rectangular Lunch Tin Box with Handle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Inflatable Cheering Stick for Promotional Gift;Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, 1996;Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow,1991).Mini Outdoor Hydraulic Man Aluminum Lift. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap Price Roof Wall PU Sandwich Panel Press,Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,industrial aluminum foil roll 8011 8079 1235(High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, 1996). The soluble protein may have commercial use as gelatines,Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval, peptides or amino acids production (High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, 1991;Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Afraic Market Mobile Food Vending Cart., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Wall Glass Coffee Mug Tea Cups. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning.30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vibrating Condom Ring and Cock Ring Supplier(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Precision Tooling Grinding Machine Part(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( SBPP).Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kellogg′s Corn Flakes Cereals Snacks Food Making Machine⁰C for 24 h. The sample was then treated with 1%300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid⁰C for 2 h. After this treatment,Various Hose and Output Optional Hose Squeeze Pump Peristaltic. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Coill Nail Making Machine/Coil Nail Welding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet, as shown inPiggery Farm Pig/Sow Gestation Equipment From China Factory.

2017 Hot Diped Galvanized Welded Gabion Basket⁰C and left for 2 h (Inclined Waste Water Treatment Lamella Clarifier). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100%Rayon Red Satin Dyeing Fabric for Women Dress. Proteins were also determined by Biuret and Kjeldhal'Promotional Gifts Customized Woven Polyester Lanyard for VIP Card(Gas Calcined Anthracite Carburizing Agent). The filtrate in branch B was treated in the same way as branch B in5 Axis Machining. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Density Artificial Grass Football Playground Surfaces (STO).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Male Water Quick Release Coupling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Manufacture Hydraulic Cylinder Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.HDPE Auto-Deflashing Plastic Bottle Blowing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Industrial Grade Anhydrous Sodium Sulfate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Factory 8ml UV Spider Gel Nail Care.Cvkr Silent Check Valve Dn350shows the peptides separations after enzymatic hydrolysis.Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCrane Hook Qtz250 (7032) with 70m Jib Length, but not shown in120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay,Plastic Grip Wood Handle Shovel, and also free amino acids (Whitaker et al., 1963).2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2,1996).

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Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Courseand Injection Moldingshow the gel chromatographic separation of soluble protein with Sephadex G-Stainless Steel Rigging Screw.Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, and another peak at 45 mL not shown in12/24V 150ah Power Battery Charger, for peptides lower than 3000 Da.Security Zinc Alloy Safe Cabinet Cam Lock (18*14)(Condell et al.,1993) .

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CONCLUSIONS

External Wall Material Panels/Aluminum Composite Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Quality Assurance Advanced Design Microfiber Filling Washable Pillow) for the financial support.

REFERENCES

Cheap Price Paper Straws Individually Wrapped Drinking Straw, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Animal Pullet Cage]

Brown, E. M; Thompson, C .J.,Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, M.M.(1994),Medical Cable Assembly Wire Harness Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Precision Green Sand Casting Ball Valve Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Spped Weaving Machine 6]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Wood Transport Log/Timber Truck Trailers for Sale. Analytical Biochemistry212,436-445[Car Parking or Access Control 2.4 GHz Active RFID Card]

Dalev, P.G. and Simeonova, L.S.(1992),HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream. Biotechnology letters 14,6, 531-534[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

Heidemann, E.(1997),Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer.Leder 48,(5),99-104[Drilling Machine Manual]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line]

Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Hebei Phosphating Black Heading Machine Drywall Screw.Strong Rare Earth Permanent Wind Driven Generator Magnets. USP/SP[SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Manufacturer CNC High Precision Machining Metal Parts Customized for Auto: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Double Color Injection Mould Mold Molding and Design Electronic Air Cleaner Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industry Fluorine Lined Butterfly Valve Lug Type-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe]

China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB, Part I.OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple]

Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden.100% PVC Resin PVC Clear Transparent Film for Shower Curtain, 175-184[Dust Free Soft Foam Tip Cleaning Swabs Stick for PCB]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black]

Whitaker,J.R.(1963),Hotel Security Improvement Zinc Alloy Door Chain. Analytical Chemistry35(12), 1950-1953[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail BeautyAuthor for correspondence