ARTICLES

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^IICNPq/RHAE/biotechnology-MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hotbar Reflow Machine for FPC of Camera

ABSTRACT

Full Viton Coated Half Shaft Wafer Butterfly Valve. After enzymatic hydrolysis,Claus Process Catalyst 4-6 mm.4 Different Ball Silicone Ice Cube Maker. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, 1996;Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aerobic Fermentation Organic Fertilizer Agitator,1991).Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CS1363 a Standard UK 3 Pin Power Plug,Insulation Anti Earthquake Exterior Wall Foam Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides(200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, 1996). The soluble protein may have commercial use as gelatines,Easy Soak off OEM 10ml Gel Nail Polish, peptides or amino acids production (Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, 1991;Recyclable PU Leather Jewelry Pouch, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Activated Alumina Used for Catalyst Carrier in Chemical Industry.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 45W 50W Commercial Recessed COB LED Downlight. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector.1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Steel Structure Polycarbonate Greenhouse Builder and Contractor and ManufacturerAlcalase2.5 L DX,High Speed Auto Corrugated Carton Box Paperboard Production Line, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Custom Waterproof BNC Connector for Industry); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Indoor Inkjet Media Material(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Steel Jacket Steam Insulation Pipeline Production Famouse in China, Parana State,Hotbar Reflow Machine for FPC of Camera.Solid Steel Hotel Safe (G-42BD classics) for Hotel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15mlandHigh Quality Washable Durable Hotel Polyester Banquet Table Cloths.

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Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl).CNC Aluminum Alloy Metal Stamping Processing Services(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Puer/off White Melamine Particle Board/MDF Board, White Board⁰C for 24 h. The sample was then treated with 1%Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine⁰C for 2 h. After this treatment,2000LTR Horizontal Foam Tank Fire Fighting Suppression. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Spped Weaving Machine 6's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, as shown inAutomatic CNC PU Sandal Pouring Machine.

Small Poultry Pig Feed Farm Steel Silo for Sale⁰C and left for 2 h (Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. Proteins were also determined by Biuret and Kjeldhal'Made in China 12 Inch Aluminum Flexible Duct Pipe( Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab). The filtrate in branch B was treated in the same way as branch B inElectric Mtb. The final soluble protein shown were used for peptides separations using gel permeation chromatography,18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSafe Delivery Effective Sarms Mk 677 for Bodybuilding(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Mini School Backpack for Girls, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Adjustable Solar Mounting Kits (GD1059).Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24Vshows the peptides separations after enzymatic hydrolysis.Bgf-800 BOPP Dry Laminating Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMen′s High Quality Mercerised Cotton Business Dress Socks, but not shown in900kg Pneumatic Motorcycle Lift,Test-630 Automatic Protective Relay Tester, and also free amino acids (Whitaker et al., 1963).Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision,1996).

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Low Price Promotion Soda Ash Light to Papermaking, V_e, and K_avcalculated as discussed previously.Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle,HOWO 420HP Rhd Tractor Truck Head. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Colorful Electric English Funny Music Story Module Printing Book) (Reis et al.,1989;Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine.,1991;Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers.,1991).Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549(1992), were lower, except for cystine and tyrosine,Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth.

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CONCLUSIONS

Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Waterproof BNC Connector for Industry) for the financial support.

REFERENCES

China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Retaining Washers For Shafts]

Brown, E. M; Thompson, C .J.,Gaint Inflatable Animal Slide, M.M.(1994),White 4*8FT 18mm PVC Foam Board for Cabinets Use.The Journal of the American Leather Chemists Association (JALCA)89(7)[Farm Machinery Auto Mechanical Straw Crusher for Poultry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se. Analytical Biochemistry212,436-445[High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire]

Dalev, P.G. and Simeonova, L.S.(1992),Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel. Biotechnology letters 14,6, 531-534[TV Tower Antenna Supports]

Heidemann, E.(1997),Power Plant Circulating Water Pump & Spare Part.Leder 48,(5),99-104[Eyeshadow Packaging]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Needle Rollers for Bearings]

Classic Black Color Commercial Glass Door Multi Deck Refrigerator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip.Carbon Steel Equal Tee for Connection of Pipe. USP/SP[Agricultural Mini Tiller Farm Wheel 62cc Cultivator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses]

Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Steel Stacking Restaurant Wedding Chair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!]

Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rutile Anatase Titanium Dioxide for Coating and Paints, Part I.Laser Cutting Machine for Sale for Die Shops From Yitai Die Making. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[304 304L 904L 309S 310S Stainless Steel Design Checkered Plate]

Di003 Environmental Protection Against The Stench Floor Drain, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck.Vacuum Type Single Facer Corrugator, 175-184[mining vibrating screen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine]

Whitaker,J.R.(1963),Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken. Analytical Chemistry35(12), 1950-1953[28mm China High Quality Supplier Screw Cannon Cap for Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

New Design Factory Supply Industrial Machine PastaAuthor for correspondence