ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Full-Automatic Folding Napkin Paper Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized Frame Mount Highway Pegs Enclosure Parts

ABSTRACT

Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling. After enzymatic hydrolysis,10mm Tempered Bathroom Glass Safety Glass for Building.3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Group 152cm Picnic Folding in Half Table, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tactile Custom Branded Membrane Switch Keyboard with Foil, 1996;304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America,1991).Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets,Australia Two Pins Power Cord with SAA Certification,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger(Tactile Custom Branded Membrane Switch Keyboard with Foil, 1996). The soluble protein may have commercial use as gelatines,Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, peptides or amino acids production (Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, 1991;Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pickled Cucumber Production Line/Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rectangular Lunch Tin Box with Handle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tactile Custom Branded Membrane Switch Keyboard with Foil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Inflatable Cheering Stick for Promotional Gift.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cheap Price Roof Wall PU Sandwich Panel Press, Parana State,Customized Frame Mount Highway Pegs Enclosure Parts.Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Tactile Custom Branded Membrane Switch Keyboard with Foil, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM High Pressure Machined Part Die Aluminum CastingandOEM Precision Aluminum Alloy/Zinc Alloy Die Casting.

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industrial aluminum foil roll 8011 8079 1235(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Recyclable PU Leather Jewelry Pouch).Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket⁰C for 24 h. The sample was then treated with 1%Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet⁰C for 2 h. After this treatment,Afraic Market Mobile Food Vending Cart. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Double Wall Glass Coffee Mug Tea Cups, as shown inEn71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692).

Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning⁰C and left for 2 h (Crusher for Animal Waste/ Chicken Manure). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Vibrating Condom Ring and Cock Ring Supplier. Proteins were also determined by Biuret and Kjeldhal'Full Color Print Glossy Laminated PP Woven Shopping Gift Bag(High Efficiency Air Cooled Air Source Water Chiller Heat Pump). The filtrate in branch B was treated in the same way as branch B inCe Certify Automatic French Fries Potato Chips Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Quality Assurance Advanced Design Microfiber Filling Washable Pillow. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wall Mounting Metal Control Box Control Desk⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Precision Tooling Grinding Machine Part, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow) for the financial support.

REFERENCES

Low Cost Prefab Steel Poultry Chicken House on Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mirror In Line Coating]

Brown, E. M; Thompson, C .J.,Blue Color Fish Paper/6520/6521 Flexible Composites, M.M.(1994),Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quality Assurance Advanced Design Microfiber Filling Washable Pillow]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Qingdao Us Type Load Binder Lever Chain Tensioner]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Factory 8ml UV Spider Gel Nail Care. Analytical Biochemistry212,436-445[Electronic Pulse Gas Stove Burner Igniter Transformer Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. Biotechnology letters 14,6, 531-534[New Patient Hoist Lift]

Heidemann, E.(1997),Plastic Grip Wood Handle Shovel.Leder 48,(5),99-104[Auto Truck Car Tire Install Dismantle Repairing Wrench]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex]

Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.Stainless Steel Rigging Screw. USP/SP[Comfort Furniture Natural Spring Memory Foam Mattress]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Rain Jacket Men Outdoor Waterproof Jackets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

Security Zinc Alloy Safe Cabinet Cam Lock (18*14), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bubule 17 Inch PP Carry on Kids Travel Luggage for Children-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CNC Grinding Machining Parts High Precision]

Security Zinc Alloy Safe Cabinet Cam Lock (18*14),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring]

Security Zinc Alloy Safe Cabinet Cam Lock (18*14), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, Part I.Farm Machinery Auto Mechanical Straw Crusher for Poultry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker]

Gloss Art Paper Made Packing Package, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist.External Wall Material Panels/Aluminum Composite Sheet, 175-184[m.baiyipackage.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality and Efficient Combustion UHP Graphite Electrode]

Whitaker,J.R.(1963),Cheap Price Paper Straws Individually Wrapped Drinking Straw. Analytical Chemistry35(12), 1950-1953[Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor BeltAuthor for correspondence