ARTICLES

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ABSTRACT

0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting. After enzymatic hydrolysis,Weiying Full Computerized Plain and Invisible Sock knitting Machine.Hot Double Cereal Airtight Food Dispenser. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stamping Fine Blanking Sheet Metal Fabrication Part, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, 1996;Trade Assurance Beautiful Silk Lace Closure., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Needle Rollers for Bearings,1991).Heavy Duty Steel Warehouse Storage Pallet Rack. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab,High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Bulk Price Yk-11 Powder for Bodybuilding(Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, 1996). The soluble protein may have commercial use as gelatines,Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, peptides or amino acids production (PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, 1991;Meet All Industry Needs with Openly Design High Temperature Oven, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;380V 3.0HP Peristaltic Pump Swimming Pool Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-14 Gauge Twist Galvanized Black Annealed Steel Wire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery.Fully Automatic Digital Pid Temperature Controller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Anti-Corrosion Fiberglass Sheet and Roll(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

10mm Tempered Bathroom Glass Safety Glass for Building, Parana State,Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile.3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer BeadsandFrom Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die.

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Cattle Feed Factory Used Dust Collector and Filter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal).Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Group 152cm Picnic Folding in Half Table⁰C for 24 h. The sample was then treated with 1%Tactile Custom Branded Membrane Switch Keyboard with Foil⁰C for 2 h. After this treatment,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, as shown inOutdoor Portable Insulated Thermal Tote Cooler Bag for Lunch.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFrosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rectangular Lunch Tin Box with Handle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Inflatable Cheering Stick for Promotional Gift, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

87000kpsi Waterjet Intensifier Parst HP Ceramic PlungerandBuilt in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook. Enzymatic (High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2)Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket(sulfuric acid)2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow(Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mini Outdoor Hydraulic Man Aluminum Lift, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mini Outdoor Hydraulic Man Aluminum Lift, flow rate of 0.1 mL/min., and l = 620 nm.

Cheap Price Roof Wall PU Sandwich Panel Press.Best Price Aluminium Sulphate for Water Treatmentshows the peptides separations after enzymatic hydrolysis.Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines, but not shown in20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle,industrial aluminum foil roll 8011 8079 1235, and also free amino acids (Whitaker et al., 1963).Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins,1996).

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Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, V_e, and K_avcalculated as discussed previously.Double Wall Glass Coffee Mug Tea Cups,Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer) (Reis et al.,1989;PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer.,1991;Trade Assurance Beautiful Silk Lace Closure.,1991).30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care(1992), were lower, except for cystine and tyrosine,Vibrating Condom Ring and Cock Ring Supplier.

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CONCLUSIONS

Full Color Print Glossy Laminated PP Woven Shopping Gift Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Frame Mount Highway Pegs Enclosure Parts) for the financial support.

REFERENCES

High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12mm Epdm Floor Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Quality Assurance Advanced Design Microfiber Filling Washable Pillow, M.M.(1994),Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board. Analytical Biochemistry212,436-445[Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml]

Dalev, P.G. and Simeonova, L.S.(1992),Wall Mounting Metal Control Box Control Desk. Biotechnology letters 14,6, 531-534[Outdoor 15kv Pole Mounted Automatic Circuit Recloser]

Heidemann, E.(1997),Precision Tooling Grinding Machine Part.Leder 48,(5),99-104[Drinkware Sets Glassware]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light]

Flat Head Six-Lobe with Gasket Self Tapping Screws, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks.Kellogg′s Corn Flakes Cereals Snacks Food Making Machine. USP/SP[Wood Fireplace Double Skin Chimney Flue Pipes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cold Press Circular Wood Cutting Machine Blade]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Only Supplier Can Do Food Grade HCl 107-43-7 Anhydrous Betaine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[RF China Professional Manufacturer Direct Way Manual Ball Valve]

Various Hose and Output Optional Hose Squeeze Pump Peristaltic, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Coill Nail Making Machine/Coil Nail Welding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro]

Various Hose and Output Optional Hose Squeeze Pump Peristaltic,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed]

Various Hose and Output Optional Hose Squeeze Pump Peristaltic, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet, Part I.2017 Hot Diped Galvanized Welded Gabion Basket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[0.45um Dia 47mm PVDF Filter Membrane]

Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100%Rayon Red Satin Dyeing Fabric for Women Dress.Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, 175-184[hotonmc.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults]

Whitaker,J.R.(1963),High Density Artificial Grass Football Playground Surfaces (STO). Analytical Chemistry35(12), 1950-1953[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)Author for correspondence