ARTICLES

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ABSTRACT

High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb. After enzymatic hydrolysis,Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults.Document Storage Durable Metal Wire Mesh Decking for Pallet Rack. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (19m Mobile LED Display Billboard for Advertising Screens, 1996;Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Replica Car Wheels with 20inch for BMW Car,1991).SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cooling Tower Replaced Fill Media,A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear(19m Mobile LED Display Billboard for Advertising Screens, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Expanded Mesh, peptides or amino acids production (Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, 1991;Lf + Hf Contactless Smart Hybrid Card, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Fairy Star Light for All Kinds of Decoration Skylike.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (19m Mobile LED Display Billboard for Advertising Screens, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Linear Vibrator Screener, for Granule and Powder Selection and Classification.Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Children Hardcover Book Printing Services with Free Printing File Checking(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily LiquidAlcalase2.5 L DX,Global Brands 10 Year Cheaper Hook & Loop Tape Male Side, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Large Size Stone Aluminum Honeycomb Panel for Facade(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector).Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging⁰C for 24 h. The sample was then treated with 1%Making Machine for EVA Soft Hose⁰C for 2 h. After this treatment,Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Efficiency Air Cooled Air Source Water Chiller Heat Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, as shown inFast Install Polyurethane Decorative Wall Panel for Homes.

12oz 16oz Glass Mason Jar with Handle and Straw⁰C and left for 2 h (Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mini Freeze Dryer for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber. Proteins were also determined by Biuret and Kjeldhal'Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure(Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)). The filtrate in branch B was treated in the same way as branch B in27.5inches Aluminum Mountain Bicycle MTB Frame with Bb68mm Threaded. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality and Efficient Combustion UHP Graphite Electrode. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGraphite Ring Good Lubrication(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins.China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lampsshows the peptides separations after enzymatic hydrolysis.Trade Assurance Beautiful Silk Lace Closure(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light, but not shown inUV Resistant Durable HDPE Reinforced Geomembrane HDPE,High Quality Needle Rollers for Bearings, and also free amino acids (Whitaker et al., 1963).Heavy Duty Steel Warehouse Storage Pallet Rack. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (19m Mobile LED Display Billboard for Advertising Screens,1996).

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Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED, V_e, and K_avcalculated as discussed previously.PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer,Meet All Industry Needs with Openly Design High Temperature Oven. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wedding Tent Cooling System Outdoor Event Air-Con Units) (Reis et al.,1989;Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2.,1991;Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042).,1991).380V 3.0HP Peristaltic Pump Swimming Pool Pump(1992), were lower, except for cystine and tyrosine,Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts.

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CONCLUSIONS

14 Gauge Twist Galvanized Black Annealed Steel Wire. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw) for the financial support.

REFERENCES

2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Professional Superior Performance A4 Copy Paper Cutter/Guillotine with Microcomputer Control System]

Brown, E. M; Thompson, C .J.,Fully Automatic Digital Pid Temperature Controller, M.M.(1994),High Quality Anti-Corrosion Fiberglass Sheet and Roll.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Viton Coated Half Shaft Wafer Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter. Analytical Biochemistry212,436-445[Nail Stamping Plates Custom Metal Part Sheet Metal Stamping]

Dalev, P.G. and Simeonova, L.S.(1992),Full-Automatic Folding Napkin Paper Machine. Biotechnology letters 14,6, 531-534[Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat]

Heidemann, E.(1997),Customized Frame Mount Highway Pegs Enclosure Parts.Leder 48,(5),99-104[Extra Good Quality Itftype 3 Standard 3 PCS or 4 PCS in One Pressurized Tube Packed Custom 57% Wool Paddle Ball]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Top Furniture Cheap Iron Stacking Banquet Hall Chairs, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling.10mm Tempered Bathroom Glass Safety Glass for Building. USP/SP[Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Design Many Styles Fancy Wine Display Rack (6532)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gasoline Stave Pickaxe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer]

Cattle Feed Factory Used Dust Collector and Filter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[European Hotel Decorative Natual Shell Square Standing Floor Lamp]

Cattle Feed Factory Used Dust Collector and Filter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[4 Gang 1 Way Electrical Fitting Ce Certificate]

Cattle Feed Factory Used Dust Collector and Filter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Group 152cm Picnic Folding in Half Table, Part I.Tactile Custom Branded Membrane Switch Keyboard with Foil. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale High Speed Mixer Blender for Extruder Machine]

304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America.Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, 175-184[SAE Flange 6000psi Bellows Flange Hose Fitting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old]

Whitaker,J.R.(1963),Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. Analytical Chemistry35(12), 1950-1953[PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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