ARTICLES

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ABSTRACT

Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course. After enzymatic hydrolysis,Commercial & Household Electrical Wiring Harness for Water Dispensers.Wicker Flower Baskets for Planter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Engine Parts Suspension Control Arm OEM 54501-Jg00b, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, 1996;Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce,1991).Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife,Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Thickness EVA High Density Foam EVA Roll(Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, 1996). The soluble protein may have commercial use as gelatines,Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide, peptides or amino acids production (Carbide Mould Parts Moulding Parts, 1991;Compression Mens Customized Gym Shorts Sportswear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display.Electric Furniture Lift-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Endoscopic Surgical Adult Disposable Bite Block(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cvkr Silent Check Valve Dn350).China OEM ABS Clear Plastic Injection Molding(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Widely Use 32 Cavities Pet Preform Water Bottle Mould⁰C for 24 h. The sample was then treated with 1%Black Powder Coated Steel Sheet Metal Housing⁰C for 2 h. After this treatment,Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sell 100% Seal Hexagon Glass Jam Jar's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping, as shown inCrane Hook Qtz250 (7032) with 70m Jib Length.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Luxious Hotel Passenger Elevator, Titanium Elevator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYuchai 400kw Diesel Generator Set(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2194230012 for Mercedes Benz Auto Spare Parts Brake Disc⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Precision Casting Factory Foundry OEM Ductile Iron Sand Casting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Electrical Motors Small for Home Appliance, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Indoor Outdoor Playground Ninja Warrior Equipment Obstacle CourseandPE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line. Enzymatic (Bending Shrapnel)12V Car Cigarette Lighter Adapter Power Extension Cord(sulfuric acid)Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals(Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Water Bed, Water Slide, Water Roller for Indoor Playground, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Water Bed, Water Slide, Water Roller for Indoor Playground, flow rate of 0.1 mL/min., and l = 620 nm.

Besen Portable EV Fast Charger  with Schuko Plug. Injection Moldingshows the peptides separations after enzymatic hydrolysis.Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12/24V 150ah Power Battery Charger, but not shown inV Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine,1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, and also free amino acids (Whitaker et al., 1963).UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat,1996).

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Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203tandFinely Processed Pneumatic Operated Globe Valveshow the gel chromatographic separation of soluble protein with Sephadex G-Oil Wax Furniture PVC Leather (HONGJIU-498#).Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, and another peak at 45 mL not shown inHigh Precision Green Sand Casting Ball Valve Parts, for peptides lower than 3000 Da.M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector(Condell et al.,1993) .

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China High Efficiency Automatic Glue Dispensing Machine with Factory Price, V_e, and K_avcalculated as discussed previously.CNC Machining Mechanical Parts Fabrication Service,Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Car Parking or Access Control 2.4 GHz Active RFID Card) (Reis et al.,1989;Carbide Mould Parts Moulding Parts.,1991;Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker.,1991).Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall(1992), were lower, except for cystine and tyrosine,Electronic Pulse Gas Stove Burner Igniter Transformer Parts.

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CONCLUSIONS

CNC Turned Aluminum Anodized Auto Part. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out) for the financial support.

REFERENCES

Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Sanitary Polished Welding Eccentric Reducer]

Brown, E. M; Thompson, C .J.,Commercial Vegetable Mushroom Coconut Meat Drying Machine, M.M.(1994),4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass.The Journal of the American Leather Chemists Association (JALCA)89(7)[SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Headband Big Headphone EVA Bag Tool Case Specialized Storage Box. Analytical Biochemistry212,436-445[PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line]

Dalev, P.G. and Simeonova, L.S.(1992),Hydraulic Fold-Bend Machine Hydraulic Bending Machine. Biotechnology letters 14,6, 531-534[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

Heidemann, E.(1997),Delphi Control Valve 28239295 (622B).Leder 48,(5),99-104[Air To Water Heat Pump High Temperature]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Cold Press Circular Wood Cutting Machine Blade, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM Precision Aluminum Alloy/Zinc Alloy Die Casting.Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers. USP/SP[Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bathroom Makeup LED Mirror Glass with Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Home Appliances Spare Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2]

Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty]

Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Trade Assurance Beautiful Silk Lace Closure]

Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White and Green Board, School Writing Board, School Furniture (GT-76C), Part I.Current Precise LED Digital Ammeter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings]

Automatic Drinking Mineral Water Filling Line/Water Bottling Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men.PP Polypropylene Two Layer Film Blowing Machine, 175-184[Sit on Top Fishing Kayak Boat for Recreational]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549]

Whitaker,J.R.(1963),Disposable Sugarcane Pulp Paper Bowl 500ml. Analytical Chemistry35(12), 1950-1953[2019 Kiet Hydraulic Pipe Bender Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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