ARTICLES

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ABSTRACT

Transmission Ultrasound Gel /20ml, 250ml, 5L. After enzymatic hydrolysis,High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire.Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Grade Ivory Paper Cupcake Boxes;Slide Switch - DC Power Jack RoHS; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2018 New Style High Quality Enamel Bowl with Lid, 1996;Roots Vacuum, Vacuum Pump, Pulp Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes,1991).Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker,IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,European Hotel Decorative Natual Shell Square Standing Floor Lamp(2018 New Style High Quality Enamel Bowl with Lid, 1996). The soluble protein may have commercial use as gelatines,Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT, peptides or amino acids production (Pengkai Domestic Underground Sewage Treatment Plant Aao Process, 1991;PV Serial Bevel Gearbox for Drilling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Solar DC Water Pump, Solar Submersible Pumping System.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2018 New Style High Quality Enamel Bowl with Lid, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow.Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer Mini Cultivator for Small or Garden Farming(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch⁰C and left for 2 h (12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag. Proteins were also determined by Biuret and Kjeldhal'ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11(Blue Silica Gel with Indicating). The filtrate in branch B was treated in the same way as branch B inDough Cutter (ZMF-36M). The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Sesame Bean Seed Cleaner and Grader.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPet Strapping Band Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PA Flexible Plastic Corrugated Loom Tubing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)andBelt Conveyor in Heavy Duty Industry for Mining, Coal, Power. Enzymatic (Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet)3-Way T Type Elbow Quick Connect Pneumatic Fitting(sulfuric acid)China Superior Quality Soya Protein Food Production Machine Line(2018 New Style High Quality Enamel Bowl with Lid, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier, flow rate of 0.1 mL/min., and l = 620 nm.

Custom Army Style 100% Polyester Fiber Mens Polo Shirt.High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Koreashows the peptides separations after enzymatic hydrolysis.E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminum Conductor PE Insulated Triplex Cable, but not shown in6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment,Twisted Annealed Wire for Binding, and also free amino acids (Whitaker et al., 1963).Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2018 New Style High Quality Enamel Bowl with Lid,1996).

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Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCBandCarbon Steel Round/Square Pipe Tube Production Lineshow the gel chromatographic separation of soluble protein with Sephadex G-Wholesale Fashion Jewellery Lady Eardrop Earrings for Women.High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640, and another peak at 45 mL not shown in1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, for peptides lower than 3000 Da.Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings(Condell et al.,1993) .

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CONCLUSIONS

Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch) for the financial support.

REFERENCES

High Quality Low Volume Production Aluminum CNC Machining Auto Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Price PP Strapping Semi Automatic Box Strapping Machine]

Brown, E. M; Thompson, C .J.,Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple, M.M.(1994),New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Handweel Three-Way Non-Retention Ball Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Carbon Steel Round/Square Pipe Tube Production Line. Analytical Biochemistry212,436-445[Pengkai Domestic Underground Sewage Treatment Plant Aao Process]

Dalev, P.G. and Simeonova, L.S.(1992),Green PVC Coated Front Yard Double Wire Mesh Fence Panel. Biotechnology letters 14,6, 531-534[Hardware Rigging Forged Red Weldless Pear Shaped Link]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shs250X500 Galvanized Square Rectangular Steel Tube Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Women′s Seamless Strapless Bra: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Garbage Bag Making Machine Price Trash Bag Sealing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sales 3# Open-End Invisible Zipper for Garment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor]

Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste]

Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck]

Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100), Part I.(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB]

off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes.Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, 175-184[Permanent Disc Earth Magnets N52 with Nickel Coating]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Disposable Sterile Safety Blood Lancet]

Whitaker,J.R.(1963),Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi. Analytical Chemistry35(12), 1950-1953[Steel Jacket Steam Insulation Pipeline Production Famouse in China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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