ARTICLES

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ABSTRACT

Pipe Connection Types of Pressing Scaffolding Universal Clamp. After enzymatic hydrolysis,Ml33-2mm Eddy Current Linear Position/Displacement Sensor.Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine, 1996;19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Dyed Colored Sand, Natural Coloured Sand,1991).China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Various Bridge NdFeB Motor Magnets with High Performance,White Melamine Powder for Industrial Uses,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,UV Resistant Durable HDPE Reinforced Geomembrane HDPE(10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine, 1996). The soluble protein may have commercial use as gelatines,2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis, peptides or amino acids production (Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch, 1991;PE Film Blowing Machine (SJ-1000-1300-1500), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.304 304L 904L 309S 310S Stainless Steel Design Checkered Plate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2017 Factory Directly Oversized Women Sunglasses.High Quanlity PTFE Thread Seal Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fiberglass Grating, FRP Grating, GRP Grating(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pet and PP Geobag with UV High Strength Thermal(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Handcraft Wood Jewelry Storage Box with Lock and Key).3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Magnetic Latching Relay for Health Life⁰C for 24 h. The sample was then treated with 1%More Cavities Plastic Mould Design⁰C for 2 h. After this treatment,Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex, as shown inEnvironmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall.

Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X⁰C and left for 2 h (Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Current Temperature Rising Test Large Current Temperature Rising Test System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Animal Manure Fertilizer Fermentation Machine. Proteins were also determined by Biuret and Kjeldhal'Compact New Design IP65 Toggle Limit Switch(Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring). The filtrate in branch B was treated in the same way as branch B inGreenhouse Darkening Shade Curtains Energy Saving Climate Thermal Screens for Chrysanthemum. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Teflon for Industrial Plastic Gasket Seal with Good Quality.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Double Walled Stainless Steel Insulated Water Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Competetive Price Tension Springs for Stapler, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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9mm/12mm Seven Color Exposed Luminous Pixel String LED, V_e, and K_avcalculated as discussed previously.Construction Scaffolding Steel Galvanized Acrow Prop,3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (10mm Cubes N52 NdFeB Block Magnets for Electronic Components) (Reis et al.,1989;Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch.,1991;19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School.,1991).Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8(1992), were lower, except for cystine and tyrosine,Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home.

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CONCLUSIONS

AKD Wax 1895/Alkyl Ketene Dimer 1895. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier) for the financial support.

REFERENCES

22.8V 150W Medical Halogen Lamp, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Motovario Gearbox]

Brown, E. M; Thompson, C .J.,Aluminum Conductor PE Insulated Triplex Cable, M.M.(1994),Home Appliance Blender Juicer Motor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16). Analytical Biochemistry212,436-445[Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices. Biotechnology letters 14,6, 531-534[High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Indoor Inkjet Media Material]

Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price.Tractor Tine Tiller Cultivator. USP/SP[Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1080W Most Powerful LED Flood Light Fixtures]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Group 152cm Picnic Folding in Half Table]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pencil Pen Lidquid Empty Eyeliner Tube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Extra Heavy Wall Thickness Seamless Tubes]

CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard]

CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag]

CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LSR Injection Mold Tooling for Medical Nipple, Part I.Nail Stamping Plates Custom Metal Part Sheet Metal Stamping. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shengya Steel Concrete Electric Pole Mould]

Hot Sale Sports Jacket Men Outdoor Waterproof Jackets, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller.Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, 175-184[High Quality Kapa PVC Foam Board Us Sign Material]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Travel Refillable Perfume Atomizer Spray Bottle]

Whitaker,J.R.(1963),22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. Analytical Chemistry35(12), 1950-1953[White Marble]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Single Phase Digital Electric Power MeterAuthor for correspondence