ARTICLES

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ABSTRACT

Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!. After enzymatic hydrolysis,2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays.Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.120PCS/M Flexible LED Strip Tira De LED Light Strip, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring, 1996;Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage,1991).Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11,High Quality Sesame Bean Seed Cleaner and Grader,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag(Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring, 1996). The soluble protein may have commercial use as gelatines,Pet Strapping Band Production Line, peptides or amino acids production (UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label, 1991;Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PA Flexible Plastic Corrugated Loom Tubing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet.3-Way T Type Elbow Quick Connect Pneumatic Fitting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Superior Quality Soya Protein Food Production Machine Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, Parana State,Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts.Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePP Polypropylene Two Layer Film Blowing MachineandEmbossed PVC Rexine Car Seat Cover Leather.

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High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence).Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full Automatic AAC Concrete Lightweight Block Making Machine⁰C for 24 h. The sample was then treated with 1%Stainless Steel CF8/CF8m Street Elbow Pipe Fittings⁰C for 2 h. After this treatment,Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, as shown in100% PVC Resin PVC Clear Transparent Film for Shower Curtain.

Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve⁰C and left for 2 h (8L Mini Fridge Freezer for Home and Car Fridge). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Low Volume Production Aluminum CNC Machining Auto Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple. Proteins were also determined by Biuret and Kjeldhal'New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank(Pet and PP Geobag with UV High Strength Thermal). The filtrate in branch B was treated in the same way as branch B inSteel Rolling Mill Machine of Two High Openable Housing Mill. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Carbon Steel Round/Square Pipe Tube Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Green PVC Coated Front Yard Double Wire Mesh Fence Panel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCable Length 11 Gun Safety Padlock(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Women′s Seamless Strapless Bra, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Strong Rare Earth Permanent Wind Driven Generator Magnetsand300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm. Enzymatic (Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100))(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station(sulfuric acid)off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw(Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, flow rate of 0.1 mL/min., and l = 620 nm.

Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting.48V 200ah UPS Battery Backup for Telecomshows the peptides separations after enzymatic hydrolysis.Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSilo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), but not shown inOffice Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16),Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, and also free amino acids (Whitaker et al., 1963).20 Experiences on Plastic Injection Moulding Service. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring,1996).

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Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, V_e, and K_avcalculated as discussed previously.Ride on Toys Riding Animals in The Mall,Finely Processed Pneumatic Operated Globe Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Prime Gi Steel Strip Galvanized Metal Strip Price) (Reis et al.,1989;UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label.,1991;Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch.,1991).Color Coated Corrugated Galvanized Roofing Steel Plate(1992), were lower, except for cystine and tyrosine,Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.

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CONCLUSIONS

Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator) for the financial support.

REFERENCES

Precision Diamond Wire Saw Rope for Cutting Concrete Stone, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Factory Using Transfer Table Motorized Transfer Solution]

Brown, E. M; Thompson, C .J.,Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty, M.M.(1994),Blince High Torque OMR Hydro Motor for Injection Moulding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Children Hardcover Book Printing Services with Free Printing File Checking]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2017 Factory Directly Oversized Women Sunglasses]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. Analytical Biochemistry212,436-445[3.2m Vertical Wheelchair Elevator Platform for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),TV Tower Antenna Supports. Biotechnology letters 14,6, 531-534[Nordic Solid Wood Dining Chair/Smile Chair]

Heidemann, E.(1997),Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring.Leder 48,(5),99-104[m.thyaohua.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting]

Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Retractable Multi Core Cable Reel.New Xcm 120 Asphalt Mixing Plant for Sale. USP/SP[High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Test-630 Automatic Protective Relay Tester]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Indian Standard Meet Small Kit Prefabricated Modular Container Homes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cummins Engine Log Transportation Log Trailer Truck for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Activated Alumina Used for Catalyst Carrier in Chemical Industry]

Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Indoor Light Linear Light Aluminium LED Profile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink]

Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers]

Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price, Part I.Graphic 240X128 Dots Monochrome LCD Display Module. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11]

Dry & Wet Concrete Surface Metal Grinding Wing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Workbench Anti-Static Table ESD Natural Rubber Table Mat.316L 10 Micron Sintered Stainless Steel Filter Disc, 175-184[Brass Compression Fitting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage]

Whitaker,J.R.(1963),Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket. Analytical Chemistry35(12), 1950-1953[Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit SetAuthor for correspondence