ARTICLES

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ABSTRACT

Smart Big Hydroponic Indoor Home Garden Planter. After enzymatic hydrolysis,China Quality Heavy Duty Globe Control Valve.Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Suppliers Laser Marking Equipment of Fiber Laser;Bonfiglioli Ta Series Helical Gearing Gearbox; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, 1996;Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Large Stock Aerators for Shrimp Farms and Fish Pond,1991).750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Diameter Aluminum Pipe 3004,Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder(LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, 1996). The soluble protein may have commercial use as gelatines,Integrated Waterproof PVC Lamination Bathroom Wall Panels, peptides or amino acids production (Embossed PVC Rexine Car Seat Cover Leather, 1991;Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Solar Aluminum Rail Splice Connector for Rail Extention., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Grade Aluminum Fly Fishing Thermometer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Single Flute End Mill Milling Cutter Tools for Wood Milling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light.Sc Fiber Optic Fast Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Luxious Hotel Passenger Elevator).Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight⁰C for 24 h. The sample was then treated with 1%355nm UV Laser Marking Equipment for Crystal Materials⁰C for 2 h. After this treatment,Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wire Mesh Welding Size 10mm X 10mm's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)P4.75 Indoor Single Dual Color Advertising LED Display Billboard, as shown inPPR PVC Polyurethane Insulated Pipe Extrusion Production Machine.

New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65⁰C and left for 2 h (Rubber Soles Vulcanizing Machine/ Press Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plug-in Assembly Type Straight Through Joint and Insulation Joint. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235). Proteins were also determined by Biuret and Kjeldhal'High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin(LED Assembly Wave Soldering Machine Price). The filtrate in branch B was treated in the same way as branch B inNatural Latex High Density Foam Pocket Spring Mattress. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Quilting Machine for Muti- Needle Mattress Quilting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Single-Screw Plastic Extruders Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBoiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5g OPP Silica Gel Desiccant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Winch With Groove Drum, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust HoodandTruck Air Dryer cartridge 14bar. Enzymatic (Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors)3mm White Waterproof PVC Foam Board(sulfuric acid)Educational Equipment Smart Multi Touch Interactive White Board for School(LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike, flow rate of 0.1 mL/min., and l = 620 nm.

Women Party Jewelry 45cm Sequins Choker.IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camerashows the peptides separations after enzymatic hydrolysis.New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms, but not shown inHigh Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124,High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, and also free amino acids (Whitaker et al., 1963).High Freqency PCB with Blind Via. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L,1996).

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Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage, V_e, and K_avcalculated as discussed previously.Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick,Comfort Furniture Natural Spring Memory Foam Mattress. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (600mm Warehouse No Power Roof Fan) (Reis et al.,1989;Embossed PVC Rexine Car Seat Cover Leather.,1991;Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine.,1991).Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume(1992), were lower, except for cystine and tyrosine,4.2A Type a Type C Pd QC3.0 USB Charger.

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CONCLUSIONS

Ferrous Sulphate Monohydrate 30%Min Factory Wholesale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Modern Steel Structure Layer Chicken Farm House Designs) for the financial support.

REFERENCES

Small Portable Scooters Folding Electric Scooters for Adults, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Layer Vibrating Screen Rock Shaker Screen]

Brown, E. M; Thompson, C .J.,Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, M.M.(1994),DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Style Custom Logo Makeup Multi Colored Eyeshadow Palette]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle. Analytical Biochemistry212,436-445[ Stc-001/Stc-100A/Stc/8080A+]

Dalev, P.G. and Simeonova, L.S.(1992),Most Popular Split Pressurized Flat Panel Solar Water Heater. Biotechnology letters 14,6, 531-534[Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation]

Heidemann, E.(1997),Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter.Leder 48,(5),99-104[4G WiFi GPS Dual System 170 Degree Wide Angle Ambarella A12 Police Video Body Worn Camera]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files]

Small Steel Silo for Sale/Steel Hopper, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine.Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag. USP/SP[Ce Approval Biscuit Trayless Packing Machine (MZ-250B)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Commercial Vegetable Mushroom Coconut Meat Drying Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized UV Drying Heating Machine for Screen Printer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Solid Steel Hotel Safe (G-42BD classics) for Hotel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Classic Metal Crown Multi Compartments Women Wallet Purse]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carbon Steel Pipe ASTM A106 A53]

99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss]

99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector]

99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, Part I.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player]

Cog Mono Round Display LCD Module, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black.460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, 175-184[Cheap Custom Logo Printing Fabric RFID NFC Woven Silicone Wristband]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes]

Whitaker,J.R.(1963),China Products Suppliers Camlock Coupling Quick Couplings Female. Analytical Chemistry35(12), 1950-1953[Feed Storage OEM Grain Storage Silo 1cbm to 200cbm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning BrushAuthor for correspondence