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ABSTRACT

Electric Furniture Lift. After enzymatic hydrolysis,Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter.Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Construction Material Hoist Rack and Pinion;3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, 1996;2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Endoscopic Surgical Adult Disposable Bite Block,1991).China OEM ABS Clear Plastic Injection Molding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Widely Use 32 Cavities Pet Preform Water Bottle Mould,Black Powder Coated Steel Sheet Metal Housing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring(Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, 1996). The soluble protein may have commercial use as gelatines,Hot Sell 100% Seal Hexagon Glass Jam Jar, peptides or amino acids production (Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping, 1991;High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heavy Caliber Copper Rectangular Steel Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2mm White Coated Paper Cake Boards Jordan. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box.Luxious Hotel Passenger Elevator, Titanium Elevator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Yuchai 400kw Diesel Generator Set(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, Parana State,P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display.Bending Shrapnel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady DressesandLow Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!.

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12V Car Cigarette Lighter Adapter Power Extension Cord(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (304 304L 904L 309S 310S Stainless Steel Design Checkered Plate).Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Water Bed, Water Slide, Water Roller for Indoor Playground⁰C for 24 h. The sample was then treated with 1%Besen Portable EV Fast Charger  with Schuko Plug⁰C for 2 h. After this treatment,Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, as shown inCosmetics Facial Cream Toothpaste Tube Filling Sealing Machine.

Oil Wax Furniture PVC Leather (HONGJIU-498#)⁰C and left for 2 h (28mm China High Quality Supplier Screw Cannon Cap for Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector. Proteins were also determined by Biuret and Kjeldhal'China High Efficiency Automatic Glue Dispensing Machine with Factory Price(New Design Factory Supply Industrial Machine Pasta). The filtrate in branch B was treated in the same way as branch B inHigh Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Machining Mechanical Parts Fabrication Service.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEnvironmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Turned Aluminum Anodized Auto Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Commercial Vegetable Mushroom Coconut Meat Drying Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pulse Output Vortex Flow Meter with Flanged Connection Wate Meterand4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass. Enzymatic (Headband Big Headphone EVA Bag Tool Case Specialized Storage Box)Hydraulic Fold-Bend Machine Hydraulic Bending Machine(sulfuric acid)Delphi Control Valve 28239295 (622B)(Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers.Releasable/Resuable Stainless Steel Metalic Tie Strap for Bindingshows the peptides separations after enzymatic hydrolysis.Bathroom Makeup LED Mirror Glass with Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIC LED Wire Loop Height Measuring Microscope, but not shown inSingle Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump,Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, and also free amino acids (Whitaker et al., 1963).Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal,1996).

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Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men, V_e, and K_avcalculated as discussed previously.PP Polypropylene Two Layer Film Blowing Machine,Disposable Sugarcane Pulp Paper Bowl 500ml. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector) (Reis et al.,1989;Metal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping.,1991;2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses.,1991).Flat Roof Insulation Board Heat Insulation Board(1992), were lower, except for cystine and tyrosine,2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon.

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CONCLUSIONS

Custom Female Threaded Tee T Shape Pipe Fittings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision Casting Factory Foundry OEM Ductile Iron Sand Casting) for the financial support.

REFERENCES

Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Axis Cnc Lathe]

Brown, E. M; Thompson, C .J.,Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, M.M.(1994),Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker.The Journal of the American Leather Chemists Association (JALCA)89(7)[22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste. Analytical Biochemistry212,436-445[Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)]

Dalev, P.G. and Simeonova, L.S.(1992),Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools. Biotechnology letters 14,6, 531-534[Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator]

Heidemann, E.(1997),8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes.Leder 48,(5),99-104[Hotel Furniture Set Bedroom]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[LED UV Portable Toothbrush Sterilizer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Wood Transport Log/Timber Truck Trailers for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Competitive Price Welded Aluminum Sport Outboard Boats for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress]

Automatic Screw Press Dewatering Machine for Cassava Residue, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Indoor Football Soccer Goal Post Net for Kids-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminium Profile Bending Machine for Insulating Glass]

Automatic Screw Press Dewatering Machine for Cassava Residue,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box]

Automatic Screw Press Dewatering Machine for Cassava Residue, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear, Part I.20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel]

Hot Sale High Speed Mixer Blender for Extruder Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid.High Density Insulation Rock Wool Board for Wall, 175-184[Diode Lipo Laser Body Slimming Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation]

Whitaker,J.R.(1963),Garbage Bag Making Machine Price Trash Bag Sealing Machine. Analytical Chemistry35(12), 1950-1953[Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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