ARTICLES

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ABSTRACT

Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor. After enzymatic hydrolysis,Decorative Aluminum Wall Cladding Panel with Durable Painting Finish.Steel Grating for Industrial Platform. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c, 1996;Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag,1991).Lint Free Nail Gel Polish Remover Nonwoven Wipes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shs250X500 Galvanized Square Rectangular Steel Tube Price,Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe(Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c, 1996). The soluble protein may have commercial use as gelatines,Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt, peptides or amino acids production (From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die, 1991;Mini Double Hole ABS Plastic CNC Machined Parts Importer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;KZ-19/16/13 Pneumatic Combination Steel Binding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Packing Self Sealing Sterilization Pouch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Water Filter Cartridge PP Sediment Filter Cartridge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale.Disposable Skin Stapler (CSPF-35W)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12# X 14# Electric Galvanized Double Strands Barbed Wire).China Manufacturer Garbage Incinerator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China water meter factory water flow meter vane wheel⁰C for 24 h. The sample was then treated with 1%4 Heads High Speed Professional Paper Cardboard Laser Cutter⁰C for 2 h. After this treatment,Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, as shown inDump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck.

Gas Calcined Anthracite Carburizing Agent⁰C and left for 2 h (Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Silver Hammered Round Ball and Natural Stone Necklace. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape. Proteins were also determined by Biuret and Kjeldhal'New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies(Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye). The filtrate in branch B was treated in the same way as branch B in1600c Vacuum Atmosphere Oven. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12 Teeth Green Painting Steel Rake.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Car Parking or Access Control 2.4 GHz Active RFID Card. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avScrew Press Dewatering Machine for Handling The Reject From Paper Mill(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4lz-4.5 Small Tank Kubota Similar Rice Harvester⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Automatic Snack Food Lab Twin Screw Extruder Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, V_e, and K_avcalculated as discussed previously.Plastic Recycling Centrifugal Dewatering Machine,Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping) (Reis et al.,1989;From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die.,1991;Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring.,1991).EAS Anti-Theft Optical Security Tag for Glasses Bottle(1992), were lower, except for cystine and tyrosine,Single Phase Digital Electric Power Meter.

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CONCLUSIONS

Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory) for the financial support.

REFERENCES

2W 100lm LED Solar Lawn Light for Garden or Yard Lighting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Featured]

Brown, E. M; Thompson, C .J.,Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, M.M.(1994),28mm China High Quality Supplier Screw Cannon Cap for Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[4lz-4.5 Small Tank Kubota Similar Rice Harvester]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger. Analytical Biochemistry212,436-445[Stainless Steel Rigging Screw]

Dalev, P.G. and Simeonova, L.S.(1992),Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure. Biotechnology letters 14,6, 531-534[ Pipe Tube Curving Machine with Super Quality]

Heidemann, E.(1997),Nonwovenface Mask Production Line.Leder 48,(5),99-104[Sweet Dream Natural Latex Memory Foam King Size Pocket Spring Mattress]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Premium quality for Potassium Humate powder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[100%Rayon Red Satin Dyeing Fabric for Women Dress]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-HVAC Ventilation Supply Air Return Grille Air Vent: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Water Bed]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Workout Without Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin]

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High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pre Painted Steel Coil and Sheet]

High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Output Vulcanizing Press Machine, Part I.CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Frame Mount Highway Pegs Enclosure Parts]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%]

Whitaker,J.R.(1963),Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence. Analytical Chemistry35(12), 1950-1953[Global Brands 10 Year Cheaper Hook & Loop Tape Male Side]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm GdAuthor for correspondence