ARTICLES

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ABSTRACT

FKM Rubber Gasket/Rubber O Rings in China. After enzymatic hydrolysis,Inclined Waste Water Treatment Lamella Clarifier.33kv 100kVA Dry-Type Distribution Transformer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), 1996;Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Manufacturer Export Copper Foot Tap/Valve (HFV-001),1991).Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PVC Corrugated or Smooth Surface Helix Suction Water Hose,Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ivd Component PP Barbed Threaded Connector(Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), 1996). The soluble protein may have commercial use as gelatines,Workshop Tools 7.5 Ton Electric Winch with Dual Speed, peptides or amino acids production (Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, 1991;Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.RFID Blocking Leather Slim Zipper Credit Card Holder Wallet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2017 New Design Vinyl 3D Stone Wallpaper Factory in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Small Paper Gift Box with Magnet Closure for E-Cigarette.Agricultural Mini Tiller Farm Wheel 62cc Cultivator-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automotive Sheet Metal Stamping Car Auto Parts, Parana State,Rugged Construction Material for Steel Screens for Platforms and Floor Channels..Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBrown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper BoxesandDamage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500.

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Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler).2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation⁰C for 24 h. The sample was then treated with 1%New Patient Hoist Lift⁰C for 2 h. After this treatment,CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering, as shown inSauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price.

Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng⁰C and left for 2 h (Claus Process Catalyst 4-6 mm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1080W Most Powerful LED Flood Light Fixtures. Proteins were also determined by Biuret and Kjeldhal'OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery(316L 10 Micron Sintered Stainless Steel Filter Disc). The filtrate in branch B was treated in the same way as branch B inCustom Microfiber Polyester Spandex Wholesale Tan Through Swimwear Fabric. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Bucket Segment Bolt 6V0937. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Datashows the peptides separations after enzymatic hydrolysis.EPDM, PVC Car or Door Rubber Sealing Strip(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Resin Trap Strainer, but not shown inESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat,Black Color Tube Eyelet Lift Gas Spring, and also free amino acids (Whitaker et al., 1963).Safety Edge Sensor for Agv Automated Guided Vehicle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A),1996).

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CONCLUSIONS

Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124) for the financial support.

REFERENCES

Nice Quality Coloring Printing Children Board Book Printing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wq/Qw Submersible Sewage Pump]

Brown, E. M; Thompson, C .J.,Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808), M.M.(1994),Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10in 1500lb Pressure Seal Forged Steel Swing Check Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating. Analytical Biochemistry212,436-445[Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China]

Dalev, P.G. and Simeonova, L.S.(1992),Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley. Biotechnology letters 14,6, 531-534[Professional Manufacture Hydraulic Cylinder Tube]

Heidemann, E.(1997),Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen.Leder 48,(5),99-104[Iced Coffee Glasses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Foteck SSR 40da Solid State Power SSR Relay: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CS1363 a Standard UK 3 Pin Power Plug]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cabina de espray de coche]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables]

2015 New Square Folding Ajustable Wall LED Bathroom Mirror, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[on-Sale Flat Transparent Glass Jar for Sweet]

2015 New Square Folding Ajustable Wall LED Bathroom Mirror,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector]

2015 New Square Folding Ajustable Wall LED Bathroom Mirror, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:13.8cm Reed Diffuser Clear Glass Bottle for Aroma, Part I.Metal Cutting Machine CNC Plasma Cutter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[T Slot Aluminum Extrusion Ob4040b Aluminium Profile]

Medical Suction Connecting Tube with Yankauer Suction Handle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Surface Mounted Wall Light for Home Decoration Waterproof.Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant, 175-184[Smc Rectangular Manhole Cover]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Power Plant Circulating Water Pump & Spare Part]

Whitaker,J.R.(1963),Self-Propelled Wheel Farm Using 3 Rows Corn Harvester. Analytical Chemistry35(12), 1950-1953[High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chenille Microfiber Mop HeadAuthor for correspondence