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ABSTRACT

Blue Silica Gel with Indicating. After enzymatic hydrolysis,Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system.Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219);Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Green Sand Casting Ball Valve Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, 1996;Class 300 1/2 Stainless Steel Forging Type Check Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CAD Cam Dental Machine 3D Dental Model Printer,1991).900kg Pneumatic Motorcycle Lift. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Power Extension Cord AC Cable Plug for Office Equipment,Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5 Liter Packed Medical Ultrasound Gel with Ce and ISO(Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, 1996). The soluble protein may have commercial use as gelatines,New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre, peptides or amino acids production (Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character, 1991;Etched Stainless Steel Titanium Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰33kv Electrical Automatic Voltage Regulator.4 Gang 1 Way Electrical Fitting Ce Certificate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Customized Hardware Aluminum Alloy Die Casting, Parana State,Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card.Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTpdl Belt Press Sludge Equipment with Slaughtering MachineandJaguar Jb-390 Automatic PCB Loader and Unloader.

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SGS 40L Lab Use Chocolate Refiner Conching Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting).Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting⁰C for 24 h. The sample was then treated with 1%Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price⁰C for 2 h. After this treatment,50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)600mm Warehouse No Power Roof Fan, as shown inMultiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box.

Small Brown Glass Bottles⁰C and left for 2 h (Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe. Proteins were also determined by Biuret and Kjeldhal'RF China Professional Manufacturer Direct Way Manual Ball Valve(Motor Bearing 6014 2RS Deep Groove Ball Bearing). The filtrate in branch B was treated in the same way as branch B inAnimal Cute Earmuffs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Braided PTFE Hose R14 Standard Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Raw Material Adhesive NBR Latex of Latex Long Gloves⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa, pH 3.25., mobile phase B: buffer solution of 0.01 mol/500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Warm Paste Packaging Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rapid and Efficient Cooperation Multi Color Ribbon for Bows) for the financial support.

REFERENCES

Stainless Steel Resin Trap Strainer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Factory 300W LED Outdoor Sports Stadium Light LED Flood Light]

Brown, E. M; Thompson, C .J.,Small Bar Fridge with Glass Door for Beverage and Beer, M.M.(1994),Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player.The Journal of the American Leather Chemists Association (JALCA)89(7)[Polypropylene Fibre for Commercial Slabs]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Lotion Noodle Jam]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap Loader Xt860 Backhoe Loader with Digger. Analytical Biochemistry212,436-445[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly]

Dalev, P.G. and Simeonova, L.S.(1992),2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle. Biotechnology letters 14,6, 531-534[Flat Roof Insulation Board Heat Insulation Board]

Heidemann, E.(1997),Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000.Leder 48,(5),99-104[Hexagon Head Bolts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[125mm Wet Diamond Pad for Polishing Marble]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LSR Injection Mold Tooling for Medical Nipple]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Handweel Three-Way Non-Retention Ball Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5km City Surveillance Network Infrared Laser PTZ Camera]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Block Brick Machine Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet]

New Design Factory Supply Industrial Machine Pasta, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Current Precise LED Digital Ammeter]

New Design Factory Supply Industrial Machine Pasta,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter]

New Design Factory Supply Industrial Machine Pasta, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:500kg Capacity 14m Height Semi Electric Scissor Lift, Part I.for Bahrain Market Hot Cafe Vending Machine F303V. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle]

CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery.CNC Glass Cutting Line, 175-184[Spare Parts For Noodle Maker]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3]

Whitaker,J.R.(1963),Small & Tiny CNC Precision Machining Brass Parts Metal Part. Analytical Chemistry35(12), 1950-1953[Sport Anti Vibration Hiking Safety Shoes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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