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ABSTRACT

Professional Manufacture Hydraulic Cylinder Tube. After enzymatic hydrolysis,Chenille Microfiber Mop Head.HDPE Auto-Deflashing Plastic Bottle Blowing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, 1996;Low Cost Prefab Steel Poultry Chicken House on Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Blue Color Fish Paper/6520/6521 Flexible Composites,1991).Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Factory 8ml UV Spider Gel Nail Care,Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Grip Wood Handle Shovel(316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, 1996). The soluble protein may have commercial use as gelatines,2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, peptides or amino acids production (Stainless Steel Rigging Screw, 1991;Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Security Zinc Alloy Safe Cabinet Cam Lock (18*14)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bubule 17 Inch PP Carry on Kids Travel Luggage for Children.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Farm Machinery Auto Mechanical Straw Crusher for Poultry.Gloss Art Paper Made Packing Package-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack⁰C and left for 2 h (3-Way T Type Elbow Quick Connect Pneumatic Fitting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden. Proteins were also determined by Biuret and Kjeldhal'100% PVC Resin PVC Clear Transparent Film for Shower Curtain(Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape). The filtrate in branch B was treated in the same way as branch B inInstruments For Control Temperature. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hotel Security Improvement Zinc Alloy Door Chain.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anti-Pollution Type 160kn Insulator Porclelain Disc. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNickel 200 Floating Ball Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Tent Windows Use China Made PVC Transparent Clear Mesh Tarp. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml) for the financial support.

REFERENCES

13 Oz Flex Banner PVC Frontlit Banner Material, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gray Ductile Iron Sand Casting Parts for Forklift Truck]

Brown, E. M; Thompson, C .J.,Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, M.M.(1994),3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables.The Journal of the American Leather Chemists Association (JALCA)89(7)[The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Jewelry White Gold Plated Engagement Diamond Ring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chubby 163cm Fat Big Ass Female Real Sex Doll for Man. Analytical Biochemistry212,436-445[2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Unique Best Quality Copper Home Alcohol Distiller for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stable Adjustable Monitor Mounts Folding LCD Monitor Stand]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bath Towel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Meet All Industry Needs with Openly Design High Temperature Oven]

Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress]

Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Scew Weighing Scale Electronic Counting Scale]

Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Drive Travel Car Inflatable Bed, Part I.Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DC Colorful Battery Operated Small Hand Fan for Outdoor Use]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Supply Industry Grade Sodium Gluconate]

Whitaker,J.R.(1963),SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH). Analytical Chemistry35(12), 1950-1953[Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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