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ABSTRACT

Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net. After enzymatic hydrolysis,Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage.Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pickled Cucumber Production Line/Making Machine;Rectangular Lunch Tin Box with Handle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Inflatable Cheering Stick for Promotional Gift, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, 1996;Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2,1991).Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow,Mini Outdoor Hydraulic Man Aluminum Lift,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Price Roof Wall PU Sandwich Panel Press(Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, 1996). The soluble protein may have commercial use as gelatines,Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, peptides or amino acids production (industrial aluminum foil roll 8011 8079 1235, 1991;Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Afraic Market Mobile Food Vending Cart. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders.Double Wall Glass Coffee Mug Tea Cups-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Quality Assurance Advanced Design Microfiber Filling Washable Pillow, Parana State,Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%.Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3.2m Vertical Wheelchair Elevator Platform for SaleandNordic Solid Wood Dining Chair/Smile Chair.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Density Artificial Grass Football Playground Surfaces (STO)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Manufacture Hydraulic Cylinder Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chenille Microfiber Mop Head, pH 3.25., mobile phase B: buffer solution of 0.01 mol/HDPE Auto-Deflashing Plastic Bottle Blowing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Blue Color Fish Paper/6520/6521 Flexible Composites.Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturersshows the peptides separations after enzymatic hydrolysis.Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11, but not shown inCommercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage,High Quality Factory 8ml UV Spider Gel Nail Care, and also free amino acids (Whitaker et al., 1963).Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment,1996).

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Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, V_e, and K_avcalculated as discussed previously.Security Zinc Alloy Safe Cabinet Cam Lock (18*14),Bubule 17 Inch PP Carry on Kids Travel Luggage for Children. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Strong Aluminum Armrest Reclining Hydraulic Barber Chair) (Reis et al.,1989;industrial aluminum foil roll 8011 8079 1235.,1991;Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook.,1991).300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag(1992), were lower, except for cystine and tyrosine,Farm Machinery Auto Mechanical Straw Crusher for Poultry.

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CONCLUSIONS

Gloss Art Paper Made Packing Package. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full Color Print Glossy Laminated PP Woven Shopping Gift Bag) for the financial support.

REFERENCES

3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cage Clamp Terminal Block Spring Suppliers for Sale]

Brown, E. M; Thompson, C .J.,External Wall Material Panels/Aluminum Composite Sheet, M.M.(1994),Cheap Price Paper Straws Individually Wrapped Drinking Straw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mini High Temperature Clothing Labels Screen Printing Machine Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml. Analytical Biochemistry212,436-445[Metal Cutting Machine CNC Plasma Cutter]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Cable Assembly Wire Harness Manufacturer. Biotechnology letters 14,6, 531-534[Workbench Anti-Static Table ESD Natural Rubber Table Mat]

Heidemann, E.(1997),Hot Sale Wood Transport Log/Timber Truck Trailers for Sale.Leder 48,(5),99-104[a-topmachine.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Adjustable Solar Mounting Kits (GD1059)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Forged 316 Stainless Steel Marine Twisted Long D Shackle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Hebei Phosphating Black Heading Machine Drywall Screw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[22.8V 150W Medical Halogen Lamp]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Effective UVA Mosquito Killer Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies]

Strong Rare Earth Permanent Wind Driven Generator Magnets, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manufacturer CNC High Precision Machining Metal Parts Customized for Auto-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Various Hose and Output Optional Hose Squeeze Pump Peristaltic]

Strong Rare Earth Permanent Wind Driven Generator Magnets,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip]

Strong Rare Earth Permanent Wind Driven Generator Magnets, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, Part I.Industry Fluorine Lined Butterfly Valve Lug Type. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gaint Inflatable Animal Slide]

Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack.Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, 175-184[Single Screw Extrusion Line PPR HDPE EVA Pipe Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lamella Clarifier for Wastewater Treatment Plant]

Whitaker,J.R.(1963),Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden. Analytical Chemistry35(12), 1950-1953[Handcraft Wood Jewelry Storage Box with Lock and Key]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior WallAuthor for correspondence