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ABSTRACT

Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple. After enzymatic hydrolysis,New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank.Carbon Steel Round/Square Pipe Tube Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Canvas School College Backpack/Bookbags for Girls/Students/Women, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles, 1996;Women′s Seamless Strapless Bra., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet,1991).300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100),(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw(Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles, 1996). The soluble protein may have commercial use as gelatines,Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, peptides or amino acids production (Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, 1991;Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.20 Experiences on Plastic Injection Moulding Service.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wind Protection Screen. Wind Dust Netting, Wind Dust Net. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰24K Gold Rolling Paper Shine Papers Cigarette Paper.Two - Way Double Row Thrust Tapered Roller Bearings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gaint Inflatable Animal Slide).Blince High Torque OMR Hydro Motor for Injection Moulding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring⁰C for 24 h. The sample was then treated with 1%TV Tower Antenna Supports⁰C for 2 h. After this treatment,Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Retractable Multi Core Cable Reel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Xcm 120 Asphalt Mixing Plant for Sale, as shown inLamella Clarifier for Wastewater Treatment Plant.

Indian Standard Meet Small Kit Prefabricated Modular Container Homes⁰C and left for 2 h (Handcraft Wood Jewelry Storage Box with Lock and Key). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LED Indoor Light Linear Light Aluminium LED Profile. Proteins were also determined by Biuret and Kjeldhal'50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price(Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall). The filtrate in branch B was treated in the same way as branch B inSilver Aluminium Hanging Display Snap/Poster/Photo Frame Stand. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Graphic 240X128 Dots Monochrome LCD Display Module.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dry & Wet Concrete Surface Metal Grinding Wing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWorkbench Anti-Static Table ESD Natural Rubber Table Mat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Abstract Light and Shadow Metal Oil Painting for Deco, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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360 Degree Reciprocating Car Wash Machine.Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ringshows the peptides separations after enzymatic hydrolysis.Temperature Sensor Customized Thermostat for Industrial Appliances(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminum Coil with AA3003 AA3105 H14 H16 H18, but not shown inPlush Comfort Spring Mattress Cheap Price for Wholesale,Custom Die Aluminum Castings, and also free amino acids (Whitaker et al., 1963).High Quality Meat Pie Making Machine with Different Mould Shape. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles,1996).

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CONCLUSIONS

Weak Daylight Recharge All in One Solar Street Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Color Coated Corrugated Galvanized Roofing Steel Plate) for the financial support.

REFERENCES

Ybz16-150/140 Vane Steering Hydraulic Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Basin Drain With Plug]

Brown, E. M; Thompson, C .J.,2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses, M.M.(1994),3mm Indoor Wall Cladding Decoration Aluminum Composite Material.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel CF8/CF8m Street Elbow Pipe Fittings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning. Analytical Biochemistry212,436-445[Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic CNC PU Sandal Pouring Machine. Biotechnology letters 14,6, 531-534[Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal]

Heidemann, E.(1997),Glass Disc Suspension Insulators.Leder 48,(5),99-104[dryingfree.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Indoor Inkjet Media Material]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Polypropylene Fibre for Commercial Slabs: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Cabin]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1080W Most Powerful LED Flood Light Fixtures]

Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Glass Water Jet Cutting Machine (Skwj-4018A)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Group 152cm Picnic Folding in Half Table]

Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Extra Heavy Wall Thickness Seamless Tubes]

Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:U. S. Type Clevis Grab Hook, Part I.Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard]

Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Car Trunk Organizer Car Storage Organizer.Fashion Cotton Baby/Kid Hat (DH-BF332), 175-184[Electronic Sign Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag]

Whitaker,J.R.(1963),Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line. Analytical Chemistry35(12), 1950-1953[Shengya Steel Concrete Electric Pole Mould]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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