ARTICLES

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ABSTRACT

Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet. After enzymatic hydrolysis,300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm.Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station;off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, 1996;Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal,1991).20 Experiences on Plastic Injection Moulding Service. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wind Protection Screen. Wind Dust Netting, Wind Dust Net,24K Gold Rolling Paper Shine Papers Cigarette Paper,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Two - Way Double Row Thrust Tapered Roller Bearings(Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, 1996). The soluble protein may have commercial use as gelatines,Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, peptides or amino acids production (Ride on Toys Riding Animals in The Mall, 1991;Finely Processed Pneumatic Operated Globe Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Color Coated Corrugated Galvanized Roofing Steel Plate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Precision Diamond Wire Saw Rope for Cutting Concrete Stone.Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Blince High Torque OMR Hydro Motor for Injection Moulding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Xcm 120 Asphalt Mixing Plant for Sale, Parana State,Women′s Seamless Strapless Bra.Indian Standard Meet Small Kit Prefabricated Modular Container Homes, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theConcentric Reducer 3 Inch*2 Inch A105 ASME B16.9andComplete Maize Starch Wet Milling Plant with High Quality.

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Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker).LED Indoor Light Linear Light Aluminium LED Profile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price⁰C for 24 h. The sample was then treated with 1%Graphic 240X128 Dots Monochrome LCD Display Module⁰C for 2 h. After this treatment,Dry & Wet Concrete Surface Metal Grinding Wing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Workbench Anti-Static Table ESD Natural Rubber Table Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)316L 10 Micron Sintered Stainless Steel Filter Disc, as shown inHot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure.

Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket⁰C and left for 2 h (2070 4th Axis CNC). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Abstract Light and Shadow Metal Oil Painting for Deco. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003). Proteins were also determined by Biuret and Kjeldhal'Cheapest Container Living House/Office/Kiosk(China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net). The filtrate in branch B was treated in the same way as branch B inContainer Seal High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Spin Centrifugal Demister Separator or Column Internal.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Baby Wet Wipe Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClosed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.360 Degree Reciprocating Car Wash Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Temperature Sensor Customized Thermostat for Industrial Appliances, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Die Aluminum Castings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Reflective Tape for Vehicle Reflective Markings (tape-02).Outdoor Christmas Decoration Highway Street Lightshows the peptides separations after enzymatic hydrolysis.White Crystal Powder Betaine Anhydrous CAS#107-43-7(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDisposable Skin Stapler (CSPF-35W), but not shown inCNC Single Flute End Mill Milling Cutter Tools for Wood Milling,Creative New Product Coin Holder Keychain, and also free amino acids (Whitaker et al., 1963).Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting,1996).

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CONCLUSIONS

Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring) for the financial support.

REFERENCES

Polypropylene Fibre for Commercial Slabs, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[SPA Automatic Computerise UV Screen Printing Machine for PVA Plate]

Brown, E. M; Thompson, C .J.,Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, M.M.(1994),Glass Water Jet Cutting Machine (Skwj-4018A).The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Spin Centrifugal Demister Separator or Column Internal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-U. S. Type Clevis Grab Hook. Analytical Biochemistry212,436-445[Kellogg′s Corn Flakes Cereals Snacks Food Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale. Biotechnology letters 14,6, 531-534[Tongkat Ali Root Extract 200 1]

Heidemann, E.(1997),Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter.Leder 48,(5),99-104[Paper Processing Machinery Low Density Cleaner Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Barricade Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium Galvanized Corrugated Steel Roofing Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Investment Precision Casting for Valve Body with Machining]

OEM Round Stainless Steel Fruit Plate Tray, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Supply Industry Grade Sodium Gluconate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[4 Heads High Speed Professional Paper Cardboard Laser Cutter]

OEM Round Stainless Steel Fruit Plate Tray,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Tin Box for Condoms]

OEM Round Stainless Steel Fruit Plate Tray, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, Part I.En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201]

Mini High Temperature Clothing Labels Screen Printing Machine Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc..Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, 175-184[Pg571 Series Aluminium Pg Parallel Groove Clamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board]

Whitaker,J.R.(1963),Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts. Analytical Chemistry35(12), 1950-1953[5 Liter Packed Medical Ultrasound Gel with Ce and ISO]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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