ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,SGS 40L Lab Use Chocolate Refiner Conching Machine

ABSTRACT

Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine. After enzymatic hydrolysis,Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting.Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.600mm Warehouse No Power Roof Fan, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Brown Glass Bottles, 1996;Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe,1991).RF China Professional Manufacturer Direct Way Manual Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected,Stainless Braided PTFE Hose R14 Standard Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser(Small Brown Glass Bottles, 1996). The soluble protein may have commercial use as gelatines,Black Steel ASTM A192 Seamless Pipe Sch120, peptides or amino acids production (Raw Material Adhesive NBR Latex of Latex Long Gloves, 1991;Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1200X1000X810 Strong Collapsible Plastic Containers for Storage. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Brown Glass Bottles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fast Install Polyurethane Decorative Wall Panel for Homes.Customized Factory Supply Stainless Steel T Shape Bolt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Women Summer Swimsuit Chest Zip Bathing Suit, Parana State,SGS 40L Lab Use Chocolate Refiner Conching Machine.Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Small Brown Glass Bottles, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePaper or PVC Mobile or Cellphone Scratch Recharge Prepaid CardandPE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable.

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Female Header Conn Ptich 1.0mm Connector Btb(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi).Tie Down Nylon Back to Back Hook and Loop Straps(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck⁰C for 24 h. The sample was then treated with 1%42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor⁰C for 2 h. After this treatment,Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Warm Paste Packaging Machine, as shown in20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory.

Stainless Steel Resin Trap Strainer⁰C and left for 2 h (1200X1000X810 Strong Collapsible Plastic Containers for Storage). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Small Bar Fridge with Glass Door for Beverage and Beer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player. Proteins were also determined by Biuret and Kjeldhal'Cheap Loader Xt860 Backhoe Loader with Digger(2019 New Advertising Inflatable LED Jellyfish Balloon for Sale). The filtrate in branch B was treated in the same way as branch B inPC Series One Touch Quick Coupling Microduct Push in Pneumatic Fittings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLithium Battery 20ah Pouch 3c Cell 3.7V(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Handweel Three-Way Non-Retention Ball Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Design Factory Supply Industrial Machine Pasta, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Small & Tiny CNC Precision Machining Brass Parts Metal Part.China Manufacturer Garbage Incineratorshows the peptides separations after enzymatic hydrolysis.Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery, but not shown inNew Vmc Machine Auto Parts Processing CNC Vertical Machining Centre,2019 Kiet Hydraulic Pipe Bender Machine, and also free amino acids (Whitaker et al., 1963).PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Brown Glass Bottles,1996).

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CONCLUSIONS

Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Tensisle Strength Tissue Paper) for the financial support.

REFERENCES

LED Emergency Exit Light Rechargeable 220V, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[btmarkcn.com]

Brown, E. M; Thompson, C .J.,Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, M.M.(1994),Firefighting Equipment Aluminum Rescue Tripod with Winch 30m.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Coil Control Solenoid Valve of Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Small Paper Gift Box with Magnet Closure for E-Cigarette]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279. Analytical Biochemistry212,436-445[Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension]

Dalev, P.G. and Simeonova, L.S.(1992),Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer. Biotechnology letters 14,6, 531-534[Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS]

Heidemann, E.(1997),Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter.Leder 48,(5),99-104[1.8L High Quality Borosilicate Glass Storage Bottle (GB570041700)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM High Pressure Machined Part Die Aluminum Casting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Auto Parts Sheet Metal Deep Drawing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Precision Aluminum Alloy/Zinc Alloy Die Casting]

Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum/Aluminium Extrusion Profile Housing for LED Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection]

Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Recyclable PU Leather Jewelry Pouch]

Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11, Part I.Single Mode Sc/APC Simplex Fiber Optic Adapter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)]

120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine.Abrasive 6 Sanding Disc for Automotive Industry, 175-184[Galvanized Steel Ring & Hook Cow Chain]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Crusher for Animal Waste/ Chicken Manure]

Whitaker,J.R.(1963),Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine. Analytical Chemistry35(12), 1950-1953[High Efficiency Air Cooled Air Source Water Chiller Heat Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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