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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Retractable Multi Core Cable Reel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,New Xcm 120 Asphalt Mixing Plant for Sale

ABSTRACT

Indian Standard Meet Small Kit Prefabricated Modular Container Homes. After enzymatic hydrolysis,Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings.LED Indoor Light Linear Light Aluminium LED Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dry & Wet Concrete Surface Metal Grinding Wing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Workbench Anti-Static Table ESD Natural Rubber Table Mat, 1996;316L 10 Micron Sintered Stainless Steel Filter Disc., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket,1991).Abstract Light and Shadow Metal Oil Painting for Deco. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003),Cheapest Container Living House/Office/Kiosk,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Spin Centrifugal Demister Separator or Column Internal(Workbench Anti-Static Table ESD Natural Rubber Table Mat, 1996). The soluble protein may have commercial use as gelatines,Baby Wet Wipe Machine, peptides or amino acids production (Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, 1991;Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;360 Degree Reciprocating Car Wash Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Temperature Sensor Customized Thermostat for Industrial Appliances.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Die Aluminum Castings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Workbench Anti-Static Table ESD Natural Rubber Table Mat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Meat Pie Making Machine with Different Mould Shape.Gear Operated Flanged High Performance Butterfly Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3Alcalase2.5 L DX,Wholesale Universal Garden Smart Flower Pot, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Reflective Tape for Vehicle Reflective Markings (tape-02)); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)White Crystal Powder Betaine Anhydrous CAS#107-43-7(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Creative New Product Coin Holder Keychain, Parana State,New Xcm 120 Asphalt Mixing Plant for Sale.Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Workbench Anti-Static Table ESD Natural Rubber Table Mat, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSmall Poultry Pig Feed Farm Steel Silo for Saleand9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board.

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Weak Daylight Recharge All in One Solar Street Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Double Wall Glass Coffee Mug Tea Cups).Ybz16-150/140 Vane Steering Hydraulic Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses⁰C for 24 h. The sample was then treated with 1%3mm Indoor Wall Cladding Decoration Aluminum Composite Material⁰C for 2 h. After this treatment,240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic CNC PU Sandal Pouring Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Glass Disc Suspension Insulators, as shown inRelleno De Acido Hialuronico Con Enlace Cruzado Zenfil.

2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator⁰C and left for 2 h (Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Polypropylene Fibre for Commercial Slabs. Proteins were also determined by Biuret and Kjeldhal'Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module(High Quanlity PTFE Thread Seal Tape). The filtrate in branch B was treated in the same way as branch B inPerformance Fuel Injectors. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Glass Water Jet Cutting Machine (Skwj-4018A).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%U. S. Type Clevis Grab Hook. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avConstruction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Car Trunk Organizer Car Storage Organizer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Cotton Baby/Kid Hat (DH-BF332), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Oil Wax Furniture PVC Leather (HONGJIU-498#)andOEM Round Stainless Steel Fruit Plate Tray. Enzymatic (Factory Supply Industry Grade Sodium Gluconate)Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set(sulfuric acid)En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube(Workbench Anti-Static Table ESD Natural Rubber Table Mat, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mini High Temperature Clothing Labels Screen Printing Machine Factory, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mini High Temperature Clothing Labels Screen Printing Machine Factory, flow rate of 0.1 mL/min., and l = 620 nm.

Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc..Electric Winch With Groove Drumshows the peptides separations after enzymatic hydrolysis.Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in9mm/12mm Seven Color Exposed Luminous Pixel String LED, but not shown in3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts,Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, and also free amino acids (Whitaker et al., 1963).Conventional Best-Selling Household Bamboo Craft Chopsticks. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Workbench Anti-Static Table ESD Natural Rubber Table Mat,1996).

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Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet, V_e, and K_avcalculated as discussed previously.CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera,Electro Galvanized Low Carbon Straight Cut Wire. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)) (Reis et al.,1989;Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump.,1991;316L 10 Micron Sintered Stainless Steel Filter Disc.,1991).Geotextile for Roofs, Civil Applications. Railway Work(1992), were lower, except for cystine and tyrosine,Pneumatic Shuttle Valve Flange Ends.

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CONCLUSIONS

Stx825 Dual 15 Powerful PA Speaker, Speaker Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Reflective Tape for Vehicle Reflective Markings (tape-02)) for the financial support.

REFERENCES

Ce Approval Biscuit Trayless Packing Machine (MZ-250B), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.coreschina.com]

Brown, E. M; Thompson, C .J.,Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes, M.M.(1994),Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[UV Resistant Durable HDPE Reinforced Geomembrane HDPE]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click. Analytical Biochemistry212,436-445[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Dalev, P.G. and Simeonova, L.S.(1992),5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler. Biotechnology letters 14,6, 531-534[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

Heidemann, E.(1997),Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter.Leder 48,(5),99-104[m.hybonsecurity-eas.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm.White Pattern Acrylic Sheet for Decoration Produced by Senpu. USP/SP[Threaded Locking Circular Connectors M16 680]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[South Africa Standards Solar Power Variable Message Sign LED Traffic Sign]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wedding Tent Cooling System Outdoor Event Air-Con Units]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bevel Gearbox of 90m Finishing Mill-1117-08]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LC Multistage Pump Mechanical Seal (KLLC)]

SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent]

SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Viton Coated Half Shaft Wafer Butterfly Valve]

SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator, Part I.Supply 20-110mm PP Reducing Tee for Pipe Systems. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)]

5 Gallon Pure Water Bottle Non-Spill Cap, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks.7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care, 175-184[China Cast Iron Ornaments Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Nail Stamping Plates Custom Metal Part Sheet Metal Stamping]

Whitaker,J.R.(1963),Solid Carbide Single Flute End Mills Oblique Angle Cutting. Analytical Chemistry35(12), 1950-1953[Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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