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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, 1996;High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts,1991).OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Carbon Steel Pipe ASTM A106 A53,Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Peptides Powder Ghrp 2 for Muscle Building with Super Quality(Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, 1996). The soluble protein may have commercial use as gelatines,K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, peptides or amino acids production (EPDM, PVC Car or Door Rubber Sealing Strip, 1991;Black Color Tube Eyelet Lift Gas Spring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Safety Edge Sensor for Agv Automated Guided Vehicle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Design Felt Belt Type Cooling Table/Handling Extrusion System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood.Ycom USB Charger and Data Cable with Aluminum for Type C-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles).Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley⁰C for 24 h. The sample was then treated with 1%Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen⁰C for 2 h. After this treatment,5km City Surveillance Network Infrared Laser PTZ Camera. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)300 M Portable Water Well Mining Exploration Core Drilling Rig's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Foteck SSR 40da Solid State Power SSR Relay, as shown inChina OEM ABS Clear Plastic Injection Molding.

2015 New Square Folding Ajustable Wall LED Bathroom Mirror⁰C and left for 2 h ( Soda Ash Dense). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,13.8cm Reed Diffuser Clear Glass Bottle for Aroma. Proteins were also determined by Biuret and Kjeldhal'Metal Cutting Machine CNC Plasma Cutter(High Quality 45W 50W Commercial Recessed COB LED Downlight). The filtrate in branch B was treated in the same way as branch B inSWC490 Cardan Joint Universal Joint Coupling Drive Shaft Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Medical Suction Connecting Tube with Yankauer Suction Handle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Surface Mounted Wall Light for Home Decoration Waterproof. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Right Angle Worm Gearbox for Food and Beverage and Packaging Machines⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Manufacturer High Strength Carbon Steel Heavy Hex Nut, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Economy Combo Heat Press for Starter.Carbide Mould Parts Moulding Partsshows the peptides separations after enzymatic hydrolysis.Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCog Mono Round Display LCD Module, but not shown inProfessional Supplier of Aluminium Sulphate Non Ferric 16%-17%,Food Grade Beverage Clear PVC Tubing Hose, and also free amino acids (Whitaker et al., 1963).Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House,1996).

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CONCLUSIONS

Solar Collector Panel for Pool Heater. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage) for the financial support.

REFERENCES

ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pengkai Domestic Underground Sewage Treatment Plant Aao Process]

Brown, E. M; Thompson, C .J.,Aluminum Sheet Metal Case Parts for Industrial Computer, M.M.(1994),Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nickel 200 Floating Ball Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Class 300 1/2 Stainless Steel Forging Type Check Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder. Analytical Biochemistry212,436-445[Rapid and Efficient Cooperation Multi Color Ribbon for Bows]

Dalev, P.G. and Simeonova, L.S.(1992),RCA Audio Connector Male Gold-Plated Cable Jack Plug Male. Biotechnology letters 14,6, 531-534[Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken]

Heidemann, E.(1997),Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector.Leder 48,(5),99-104[jb-hardware]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Inflatable Cheering Stick for Promotional Gift]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Din94 Cotter Pin,Stainless Steel Cotter Pin]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gold Inlaid 24K Gold / Flowers]

Home Design Solid Wood Double Adult Bed Bedroom Furniture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine]

Home Design Solid Wood Double Adult Bed Bedroom Furniture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass]

Home Design Solid Wood Double Adult Bed Bedroom Furniture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns, Part I.304 Precision Professional Dimpled Stainless Steel Plate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring]

Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light.Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling, 175-184[Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Screw Press Dewatering Machine for Cassava Residue]

Whitaker,J.R.(1963),Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport. Analytical Chemistry35(12), 1950-1953[Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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