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ABSTRACT

IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment. After enzymatic hydrolysis,New Powder Spray Booth for Metal Furniture.Small Tin Box for Condoms. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.64mm Double Braid UHMWPE Tugboat Mooring Rope, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rubber Soles Vulcanizing Machine/ Press Machine, 1996;Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032,1991).AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade,UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Forged Steel Pressure Sealed Bonnet Globe Valve(Rubber Soles Vulcanizing Machine/ Press Machine, 1996). The soluble protein may have commercial use as gelatines,Stable Adjustable Monitor Mounts Folding LCD Monitor Stand, peptides or amino acids production (CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, 1991;Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2018 New Design Red-CNC-006 CNC Machining Aluminum Precision.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Factory Price Hg1 Self Adjustable Pipe Tank Rotator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rubber Soles Vulcanizing Machine/ Press Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Crawler Excavator Track Bolt 3p2274.SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic CNC Centerless Lathe for Peeling Steel Round Bar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Newest Adjustable Decline Bench Flat Weight Bench for Wholesales(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use).PVC Wood Grain Decorative Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer⁰C for 24 h. The sample was then treated with 1%LED UV Portable Toothbrush Sterilizer⁰C for 2 h. After this treatment,Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)American NEMA Straight Blade Plug 30A 125V/250V Power Plug's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)10in 1500lb Pressure Seal Forged Steel Swing Check Valve, as shown inLED Fairy Star Light for All Kinds of Decoration Skylike.

Custom Printed/Embossed Black Earring Packaging Cards⁰C and left for 2 h (Automotive Sheet Metal Stamping Car Auto Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mug Printing Machine Heat Press Promotion Brazil. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automotive OEM Copper Wire Harness and Connectors for Industry. Proteins were also determined by Biuret and Kjeldhal'Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg(Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles). The filtrate in branch B was treated in the same way as branch B insilicone waterproof watch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Iron Wire Dry Type Straight Line Wire Drawing Machine.China Suppliers Laser Marking Equipment of Fiber Lasershows the peptides separations after enzymatic hydrolysis.Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inManual Double Bridge Glass Cutting Table Machine (JFQG-2620), but not shown inHot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector,Barricade Tape, and also free amino acids (Whitaker et al., 1963).High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rubber Soles Vulcanizing Machine/ Press Machine,1996).

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CONCLUSIONS

Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting) for the financial support.

REFERENCES

304 Thick Wall Pressure Vessel Heat Exchanger Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[ESD Sticky Roller 4′′*18m]

Brown, E. M; Thompson, C .J.,High Quality Betaine Anhydrous with Best Price107-43-7, M.M.(1994),FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quanlity Colorful Aluminum Spacer/Standoff]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Extra Heavy Wall Thickness Seamless Tubes. Analytical Biochemistry212,436-445[750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle]

Dalev, P.G. and Simeonova, L.S.(1992),Shrink Sleeves Labels for Packaging Labeling. Biotechnology letters 14,6, 531-534[Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Sesame Bean Seed Cleaner and Grader]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Atg39 100W LED Flood Light Wall Light Wall Washer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CAD Cam Dental Machine 3D Dental Model Printer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Pet Bottle Blow Making Machine / Blower]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gear Operated Flanged High Performance Butterfly Valve]

Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PE Coated Paper for Salt Sugar Sachets Paper Food Grade]

Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain]

Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Quality Banded Type Tee, Part I.Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Die Aluminum Castings]

Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles.Rugged Construction Material for Steel Screens for Platforms and Floor Channels., 175-184[Trolley Veterinary Semen Analyzer Workstation for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China]

Whitaker,J.R.(1963),FKM Rubber Gasket/Rubber O Rings in China. Analytical Chemistry35(12), 1950-1953[Hot Sell 100% Seal Hexagon Glass Jam Jar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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