ARTICLES

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ABSTRACT

3mm White Waterproof PVC Foam Board. After enzymatic hydrolysis,Educational Equipment Smart Multi Touch Interactive White Board for School.Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Freqency PCB with Blind Via, 1996;Strong Aluminum Armrest Reclining Hydraulic Barber Chair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory SMD3528 60LEDs/M Flexible LED Strip,1991).Hot-Press Small Continuous Diamond Saw Blade Cutting Disc. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage,Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Comfort Furniture Natural Spring Memory Foam Mattress(High Freqency PCB with Blind Via, 1996). The soluble protein may have commercial use as gelatines,Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, peptides or amino acids production (4.2A Type a Type C Pd QC3.0 USB Charger, 1991;Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Portable Scooters Folding Electric Scooters for Adults., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Freqency PCB with Blind Via, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle.Most Popular Split Pressurized Flat Panel Solar Water Heater-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Full Automatic AAC Concrete Lightweight Block Making Machine).Cog Mono Round Display LCD Module(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black⁰C for 24 h. The sample was then treated with 1%460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric⁰C for 2 h. After this treatment,China Products Suppliers Camlock Coupling Quick Couplings Female. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3.2m Vertical Wheelchair Elevator Platform for Sale, as shown inDifferent Size of Ring NdFeB Magnet.

LC Multistage Pump Mechanical Seal (KLLC)⁰C and left for 2 h (Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wedding Tent Cooling System Outdoor Event Air-Con Units. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV. Proteins were also determined by Biuret and Kjeldhal'Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel(2017 Hot Diped Galvanized Welded Gabion Basket). The filtrate in branch B was treated in the same way as branch B inNew Products Sun5 Plus2 Manicure UV LED Nail Lamp 48W for Nail Gel Polish Curing Replace for LED CCFL Dual UV LED Nail Lamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Prime Gi Steel Strip Galvanized Metal Strip Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fireproof Aluminum Curtain Wall Panel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Adjustable Grooming Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Professtional Suppliers Yutong Golden Dragon Bus Spare Parts.Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Pricesshows the peptides separations after enzymatic hydrolysis.Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSteel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, but not shown inChina Hebei Phosphating Black Heading Machine Drywall Screw,Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, and also free amino acids (Whitaker et al., 1963).220V/50Hz Stainless Steel Ozone Generator with 5g/H. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Freqency PCB with Blind Via,1996).

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CONCLUSIONS

High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized UV Drying Heating Machine for Screen Printer) for the financial support.

REFERENCES

Drug Delivery ABS Hospital Medical Treatment Medicine Cart, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Speed Metal Hand Spinner Mini Fidget Spinner for Adults]

Brown, E. M; Thompson, C .J.,YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, M.M.(1994),Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress.The Journal of the American Leather Chemists Association (JALCA)89(7)[DC Stand Fan Motor PCBA Card 24V]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Colorful Electric English Funny Music Story Module Printing Book. Analytical Biochemistry212,436-445[Single Mode Sc/APC Simplex Fiber Optic Adapter]

Dalev, P.G. and Simeonova, L.S.(1992),2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine. Biotechnology letters 14,6, 531-534[L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm]

Heidemann, E.(1997),IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650).Leder 48,(5),99-104[High Quality Bucket Segment Bolt 6V0927]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)]

Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Tilt-and-Turn-with-Hidden-Hinge.Automatic Soft PVC Granule Used for Refrigerator Door Gasket. USP/SP[Newest Adjustable Decline Bench Flat Weight Bench for Wholesales]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Amplifier Tube Glass Vacuum Tube (833C)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-0.45um Dia 47mm PVDF Filter Membrane: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Large Diameter Aluminum Pipe 3004]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Extract Of Gentian]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12# X 14# Electric Galvanized Double Strands Barbed Wire]

Commecial Use Watch Display Cabinet for Watch Store Interior Design, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck]

Commecial Use Watch Display Cabinet for Watch Store Interior Design,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence]

Commecial Use Watch Display Cabinet for Watch Store Interior Design, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Slotted Round Head Aluminum Self Tapping Screw, Part I.Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye]

Classic Black Color Commercial Glass Door Multi Deck Refrigerator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker.Hotbar Reflow Machine for FPC of Camera, 175-184[FST600-202 4 20mA 0 10V Analog Temperature Sensor Probe PT1000 PT100]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300]

Whitaker,J.R.(1963),Full Viton Coated Half Shaft Wafer Butterfly Valve. Analytical Chemistry35(12), 1950-1953[Most Popular Split Pressurized Flat Panel Solar Water Heater]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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