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ABSTRACT

Pengkai Domestic Underground Sewage Treatment Plant Aao Process. After enzymatic hydrolysis,PV Serial Bevel Gearbox for Drilling Machine.Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V, 1996;China Manufacturer Mini Cultivator for Small or Garden Farming., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light,1991).Stainless Steel Art-Screen for Lobby. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch,Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat(Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V, 1996). The soluble protein may have commercial use as gelatines,Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, peptides or amino acids production (Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, 1991;2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Samples Free! ! ! Primary/Remelted/Refined Lead Ingots.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰120PCS/M Flexible LED Strip Tira De LED Light Strip.Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Manufacturer Garbage Incinerator).Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PA Flexible Plastic Corrugated Loom Tubing⁰C for 24 h. The sample was then treated with 1%Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl⁰C for 2 h. After this treatment,Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3-Way T Type Elbow Quick Connect Pneumatic Fitting, as shown inOEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery.

China Superior Quality Soya Protein Food Production Machine Line⁰C and left for 2 h (New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Army Style 100% Polyester Fiber Mens Polo Shirt. Proteins were also determined by Biuret and Kjeldhal'E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator(Customized Factory Supply Stainless Steel T Shape Bolt). The filtrate in branch B was treated in the same way as branch B inCone Crusher Mantle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Twisted Annealed Wire for Binding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Fashion Jewellery Lady Eardrop Earrings for Women(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full Automatic AAC Concrete Lightweight Block Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel CF8/CF8m Street Elbow Pipe Fittings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker TagandPure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding. Enzymatic (China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service)High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts(sulfuric acid)Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve(Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Low Volume Production Aluminum CNC Machining Auto Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Low Volume Production Aluminum CNC Machining Auto Parts, flow rate of 0.1 mL/min., and l = 620 nm.

Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple.Creative New Product Coin Holder Keychainshows the peptides separations after enzymatic hydrolysis.New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHsc Heavy Duty 5 Ton Chain Pulley Block, but not shown in14 Heads Double Door Multihead Weigher,Carbon Steel Round/Square Pipe Tube Production Line, and also free amino acids (Whitaker et al., 1963).Green PVC Coated Front Yard Double Wire Mesh Fence Panel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V,1996).

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Women′s Seamless Strapless Bra, V_e, and K_avcalculated as discussed previously.Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet,300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension) (Reis et al.,1989;Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!.,1991;China Manufacturer Mini Cultivator for Small or Garden Farming.,1991).Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)(1992), were lower, except for cystine and tyrosine,(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station.

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CONCLUSIONS

off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11) for the financial support.

REFERENCES

Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Cnc Machine Trader Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, M.M.(1994),Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Male Water Quick Release Coupling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal. Analytical Biochemistry212,436-445[OEM High Pressure Machined Part Die Aluminum Casting]

Dalev, P.G. and Simeonova, L.S.(1992),20 Experiences on Plastic Injection Moulding Service. Biotechnology letters 14,6, 531-534[OEM Precision Aluminum Alloy/Zinc Alloy Die Casting]

Heidemann, E.(1997),Wind Protection Screen. Wind Dust Netting, Wind Dust Net.Leder 48,(5),99-104[Power Wheels Forklift Electric]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Crusher for Animal Waste/ Chicken Manure]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3.0kw Spindle CNC Router with Certificate for Woodworking]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Efficiency Air Cooled Air Source Water Chiller Heat Pump]

Ride on Toys Riding Animals in The Mall, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Finely Processed Pneumatic Operated Globe Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[360 Degree Reciprocating Car Wash Machine]

Ride on Toys Riding Animals in The Mall,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Endoscopic Surgical Adult Disposable Bite Block]

Ride on Toys Riding Animals in The Mall, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Color Coated Corrugated Galvanized Roofing Steel Plate, Part I.Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Insulation Anti Earthquake Exterior Wall Foam Board]

Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Precision Diamond Wire Saw Rope for Cutting Concrete Stone.Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty, 175-184[Factory Wholesale Kids Tableware Anti Spill Baby Bowl]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ycom USB Charger and Data Cable with Aluminum for Type C]

Whitaker,J.R.(1963),Blince High Torque OMR Hydro Motor for Injection Moulding Machine. Analytical Chemistry35(12), 1950-1953[5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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