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ABSTRACT

Adjustable Grooming Table. After enzymatic hydrolysis,High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc.PE Coated Paper for Salt Sugar Sachets Paper Food Grade. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood;Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, 1996;Professtional Suppliers Yutong Golden Dragon Bus Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate,1991).Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,220V/50Hz Stainless Steel Ozone Generator with 5g/H,Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic(15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, 1996). The soluble protein may have commercial use as gelatines,Luxury Jewelry White Gold Plated Engagement Diamond Ring, peptides or amino acids production (Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, 1991;13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;100% Cotton Face Mask in Guangzhou., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM High Pressure Machined Part Die Aluminum Casting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-AISI 430 Ba/2b Embossed Stainless Steel Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China.Drug Delivery ABS Hospital Medical Treatment Medicine Cart-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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0.45um Dia 47mm PVDF Filter Membrane(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape).Commecial Use Watch Display Cabinet for Watch Store Interior Design(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring⁰C for 24 h. The sample was then treated with 1%Slotted Round Head Aluminum Self Tapping Screw⁰C for 2 h. After this treatment,Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Classic Black Color Commercial Glass Door Multi Deck Refrigerator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker, as shown inChinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves.

Hotbar Reflow Machine for FPC of Camera⁰C and left for 2 h (CNC Grinding Machining Parts High Precision). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Viton Coated Half Shaft Wafer Butterfly Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Claus Process Catalyst 4-6 mm. Proteins were also determined by Biuret and Kjeldhal'4 Different Ball Silicone Ice Cube Maker(Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring). The filtrate in branch B was treated in the same way as branch B inDrilling Grade High Viscosity Low Viscosity Sodium CMC Scmc Carboxymethyl Cellulose. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Washable Durable Hotel Polyester Banquet Table Cloths.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aerobic Fermentation Organic Fertilizer Agitator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Recyclable PU Leather Jewelry Pouch.High Quality and Efficient Combustion UHP Graphite Electrodeshows the peptides separations after enzymatic hydrolysis.Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDrive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price, but not shown inEp/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt,Activated Alumina Used for Catalyst Carrier in Chemical Industry, and also free amino acids (Whitaker et al., 1963).High Quality 45W 50W Commercial Recessed COB LED Downlight. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (15K High Frequency Online UPS UPS RS232/USB/Snmp Optional,1996).

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Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, V_e, and K_avcalculated as discussed previously.High Speed Auto Corrugated Carton Box Paperboard Production Line,Custom Waterproof BNC Connector for Industry. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder) (Reis et al.,1989;Free Sample Cheap Price Plastic Black Underwear Hanger for Sale.,1991;Professtional Suppliers Yutong Golden Dragon Bus Spare Parts.,1991).Indoor Inkjet Media Material(1992), were lower, except for cystine and tyrosine,Steel Jacket Steam Insulation Pipeline Production Famouse in China.

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CONCLUSIONS

Solid Steel Hotel Safe (G-42BD classics) for Hotel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine) for the financial support.

REFERENCES

Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Frequency 3yk1548 Vibrating Screen]

Brown, E. M; Thompson, C .J.,CNC Aluminum Alloy Metal Stamping Processing Services, M.M.(1994),Puer/off White Melamine Particle Board/MDF Board, White Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet Strapping Band Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine. Analytical Biochemistry212,436-445[500ml PP Sport Water Bottle with Ball]

Dalev, P.G. and Simeonova, L.S.(1992),2000LTR Horizontal Foam Tank Fire Fighting Suppression. Biotechnology letters 14,6, 531-534[Customized Thickness EVA High Density Foam EVA Roll]

Heidemann, E.(1997),High Spped Weaving Machine 6.Leder 48,(5),99-104[Gasoline Concrete Power Trowel Machine with Adjustable Seat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple]

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc.Small Poultry Pig Feed Farm Steel Silo for Sale. USP/SP[Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Roots Vacuum]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Handhold Explosives Detector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wire Mesh Welding Size 10mm X 10mm]

P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Made in China 12 Inch Aluminum Flexible Duct Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Promotional Gifts Customized Woven Polyester Lanyard for VIP Card]

P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PA Flexible Plastic Corrugated Loom Tubing]

P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, Part I.Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll]

Safe Delivery Effective Sarms Mk 677 for Bodybuilding, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),12# X 14# Electric Galvanized Double Strands Barbed Wire.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, 175-184[Beeswax Candle Making]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AISI 430 Ba/2b Embossed Stainless Steel Sheet]

Whitaker,J.R.(1963),Wholesale Mini School Backpack for Girls. Analytical Chemistry35(12), 1950-1953[Abstract Light and Shadow Metal Oil Painting for Deco]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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