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ABSTRACT

HVAC Ventilation Supply Air Return Grille Air Vent. After enzymatic hydrolysis,High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door.65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Output Vulcanizing Press Machine;CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Transmission Ultrasound Gel /20ml, 250ml, 5L, 1996;High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence,1991).Food Grade Ivory Paper Cupcake Boxes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Slide Switch - DC Power Jack RoHS,Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2018 New Style High Quality Enamel Bowl with Lid(Transmission Ultrasound Gel /20ml, 250ml, 5L, 1996). The soluble protein may have commercial use as gelatines,Roots Vacuum, Vacuum Pump, Pulp Making Machine, peptides or amino acids production (Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes, 1991;Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-European Hotel Decorative Natual Shell Square Standing Floor Lamp. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Transmission Ultrasound Gel /20ml, 250ml, 5L, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT.Pengkai Domestic Underground Sewage Treatment Plant Aao Process-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PV Serial Bevel Gearbox for Drilling Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2017 Factory Directly Oversized Women Sunglasses).Stainless Steel Art-Screen for Lobby(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch⁰C for 24 h. The sample was then treated with 1%Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women⁰C for 2 h. After this treatment,Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!, as shown in3.2m Vertical Wheelchair Elevator Platform for Sale.

2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays⁰C and left for 2 h (Nordic Solid Wood Dining Chair/Smile Chair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Samples Free! ! ! Primary/Remelted/Refined Lead Ingots. Proteins were also determined by Biuret and Kjeldhal'Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo(Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting). The filtrate in branch B was treated in the same way as branch B inPowered 120kn Disc Insulator Suspension Glass Insulator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,120PCS/M Flexible LED Strip Tira De LED Light Strip.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Portable Insulated Thermal Tote Cooler Bag for Lunch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Sesame Bean Seed Cleaner and Grader, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl.Test-630 Automatic Protective Relay Testershows the peptides separations after enzymatic hydrolysis.Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLow Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide, but not shown inActivated Alumina Used for Catalyst Carrier in Chemical Industry,Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, and also free amino acids (Whitaker et al., 1963).3-Way T Type Elbow Quick Connect Pneumatic Fitting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Transmission Ultrasound Gel /20ml, 250ml, 5L,1996).

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CONCLUSIONS

Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC) for the financial support.

REFERENCES

Full Automatic AAC Concrete Lightweight Block Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.skyhammertools.com]

Brown, E. M; Thompson, C .J.,Stainless Steel CF8/CF8m Street Elbow Pipe Fittings, M.M.(1994),Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Strong Aluminum Armrest Reclining Hydraulic Barber Chair]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service. Analytical Biochemistry212,436-445[2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price]

Dalev, P.G. and Simeonova, L.S.(1992),High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts. Biotechnology letters 14,6, 531-534[8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes]

Heidemann, E.(1997),Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve.Leder 48,(5),99-104[Electric Fireplace Insert For Existing Fireplace Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine]

Nonwovenface Mask Production Line, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Low Volume Production Aluminum CNC Machining Auto Parts.Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple. USP/SP[Mini High Temperature Clothing Labels Screen Printing Machine Factory]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Cutting Machine CNC Plasma Cutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Workbench Anti-Static Table ESD Natural Rubber Table Mat]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale High Efficiency Scc750A Water-Based Green Cutting Fluid with Factory Production]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Adjustable Solar Mounting Kits (GD1059)]

Carbon Steel Round/Square Pipe Tube Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Green PVC Coated Front Yard Double Wire Mesh Fence Panel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Security Zinc Alloy Safe Cabinet Cam Lock (18*14)]

Carbon Steel Round/Square Pipe Tube Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Forged 316 Stainless Steel Marine Twisted Long D Shackle]

Carbon Steel Round/Square Pipe Tube Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cable Length 11 Gun Safety Padlock, Part I.Canvas School College Backpack/Bookbags for Girls/Students/Women. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[22.8V 150W Medical Halogen Lamp]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies]

Whitaker,J.R.(1963),300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm. Analytical Chemistry35(12), 1950-1953[Various Hose and Output Optional Hose Squeeze Pump Peristaltic]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/StripAuthor for correspondence