ARTICLES

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ABSTRACT

Mellow OEM Plastic Pet House Ml-HP004. After enzymatic hydrolysis,12/24V 150ah Power Battery Charger.1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head, 1996;Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (9mm/12mm Seven Color Exposed Luminous Pixel String LED,1991).Construction Scaffolding Steel Galvanized Acrow Prop. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle,Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home(044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head, 1996). The soluble protein may have commercial use as gelatines,AKD Wax 1895/Alkyl Ketene Dimer 1895, peptides or amino acids production (22.8V 150W Medical Halogen Lamp, 1991;Aluminum Conductor PE Insulated Triplex Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Home Appliance Blender Juicer Motor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike.2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tractor Tine Tiller Cultivator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Nail Stamping Plates Custom Metal Part Sheet Metal Stamping, Parana State,New Fashion Diaper Bags Mummy Bag.Hot Sale Sports Jacket Men Outdoor Waterproof Jackets, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the12 Teeth Green Painting Steel RakeandHOWO 420HP Rhd Tractor Truck Head.

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Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Style Custom Logo Makeup Multi Colored Eyeshadow Palette).Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine⁰C for 24 h. The sample was then treated with 1%Small Steel Silo for Sale/Steel Hopper⁰C for 2 h. After this treatment,Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Smart Big Hydroponic Indoor Home Garden Planter, as shown in Stc-001/Stc-100A/Stc/8080A+.

China Quality Heavy Duty Globe Control Valve⁰C and left for 2 h (Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Suppliers Laser Marking Equipment of Fiber Laser. Proteins were also determined by Biuret and Kjeldhal'Bonfiglioli Ta Series Helical Gearing Gearbox(Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files). The filtrate in branch B was treated in the same way as branch B inFlexible Copper and Aluminum Busbar. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Bread Cake Burgers Bun Rusk Packaging Packing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Large Diameter Aluminum Pipe 3004, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ce Approval Biscuit Trayless Packing Machine (MZ-250B)andOEM/ODM Professional Activated Charcoal Teeth Whitennig Powder. Enzymatic (Integrated Waterproof PVC Lamination Bathroom Wall Panels)Embossed PVC Rexine Car Seat Cover Leather(sulfuric acid)Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves(044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Solar Aluminum Rail Splice Connector for Rail Extention, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Solar Aluminum Rail Splice Connector for Rail Extention, flow rate of 0.1 mL/min., and l = 620 nm.

Grade Aluminum Fly Fishing Thermometer.Commercial Vegetable Mushroom Coconut Meat Drying Machineshows the peptides separations after enzymatic hydrolysis.CNC Single Flute End Mill Milling Cutter Tools for Wood Milling(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSolid Steel Hotel Safe (G-42BD classics) for Hotel, but not shown inCarbon Steel Pipe ASTM A106 A53,Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light, and also free amino acids (Whitaker et al., 1963).Sc Fiber Optic Fast Connector. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head,1996).

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CONCLUSIONS

Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics) for the financial support.

REFERENCES

Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[130lm/W Government Projetct Module Basketball Tennis Sports Court Field Lighting Tunnel Light Spotlight Projector Reflector 300W Outdoor LED Tunnel Lighting]

Brown, E. M; Thompson, C .J.,355nm UV Laser Marking Equipment for Crystal Materials, M.M.(1994),Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wire Mesh Welding Size 10mm X 10mm. Analytical Biochemistry212,436-445[Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth]

Dalev, P.G. and Simeonova, L.S.(1992),P4.75 Indoor Single Dual Color Advertising LED Display Billboard. Biotechnology letters 14,6, 531-534[Mooncake Pastry and Plastic Packaging Box]

Heidemann, E.(1997),New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65.Leder 48,(5),99-104[Curcumin Boswellia]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Safe Delivery Effective Sarms Mk 677 for Bodybuilding]

Men Casual Short Pants Custom Quality Running Sports Wear Shorts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plug-in Assembly Type Straight Through Joint and Insulation Joint.Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235). USP/SP[IP65 Canopy LED Light Fixture 150W LED Gas Station Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Density Insulation Rock Wool Board for Wall]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dx51d Z275 Galvanized Steel Coil Price Per Kg]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports]

Quilting Machine for Muti- Needle Mattress Quilting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Single-Screw Plastic Extruders Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hotbar Reflow Machine for FPC of Camera]

Quilting Machine for Muti- Needle Mattress Quilting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Grade Beverage Clear PVC Tubing Hose]

Quilting Machine for Muti- Needle Mattress Quilting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, Part I.High Quanlity Colorful Aluminum Spacer/Standoff. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r]

5g OPP Silica Gel Desiccant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electric Winch With Groove Drum.Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, 175-184[Polyimide Flexible PCB Board FPC Board for Car]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker]

Whitaker,J.R.(1963),Truck Air Dryer cartridge 14bar. Analytical Chemistry35(12), 1950-1953[Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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